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F4759

Sigma-Aldrich

Fibronectin bovine plasma

powder, BioReagent, suitable for cell culture

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Synonym(s):
Fibronectin
EC Number:
MDL number:
eCl@ss:
42020141
NACRES:
NA.75

biological source

bovine plasma

Quality Level

sterility

sterile

product line

BioReagent

form

powder

mol wt

~450 kDa

packaging

pkg of 1 mg
pkg of 2 mg
pkg of 5 mg

technique(s)

cell culture | mammalian: suitable

surface coverage

1‑5 μg/cm2

solubility

H2O: 1 mg/mL at 37 °C (Store reconstituted solution in working aliquots at -20°C or lower.)

shipped in

dry ice

storage temp.

−20°C

Gene Information

bovine ... FN1(2807940)

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This Item
F1141F2518F0635
Fibronectin rat plasma powder, BioReagent, suitable for cell culture

Sigma-Aldrich

F0635

Fibronectin rat plasma

sterility

sterile

sterility

sterile-filtered

sterility

-

sterility

sterile

form

powder

form

solution

form

lyophilized powder

form

powder

mol wt

~450 kDa

mol wt

-

mol wt

~550 kDa

mol wt

-

packaging

pkg of 1 mg, pkg of 2 mg, pkg of 5 mg

packaging

pkg of 1 mg, pkg of 2 mg, pkg of 5 mg

packaging

pkg of 0.5 mg

packaging

pkg of 0.5 mg, pkg of 1 mg, pkg of 2 mg

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

General description

Fibronectin (FN) is a multifunctional glycoprotein, the gene of which is localized to human chromosome 2q34-36. This gene spans 75kb and contains 50 exons. It exists as two major isoforms, one soluble and one insoluble form. The former is present in plasma, whereas the insoluble form resides in tissues and extracellular matrix (ECM) of cartilage. There are around 20 different forms of FN due to alternative splicing of a variable region and two type III exons called Extra Domains A and B.
Fibronectin is a glycoprotein with multiple domains. It comprises a N-terminal, central binding domain and a heparin-binding domain. Bovine plasma fibronectin has polypeptide chains with disulfide linkage and corresponds to a molecular weight of 220 and 215 kDa.

Application

Fibronectin from bovine plasma is used for the following applications:
  • Used for coating the glass coverslips for the culture of decidual stromal cells (DEC)
  • Cell movement assays (culture plates were coated with fibronectin)
  • Cell spreading and immunofluorescence
  • Protein specificity
  • Integration of sensor films into microfluidic chips
  • Histological and Immunohistochemical (IHC) analyses
  • Used in primary cell cultures from patients with lung carcinoma

Biochem/physiol Actions

Fibronectin is a regulator of protein scaffolding. It coordinates cellular processes like platelet functions, wound-healing, adhesion, migration and aggregation.

Caution

As supplied, this product should be stored at -20°C and will remain active for at least 2 years.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Articles

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Protocols

Fibronectin Coating Protocol

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.

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