F4883

Fibrinogen from human plasma

35-65% protein (≥90% of protein is clottable).

• Synonym(s): Factor I
• CAS Number: 9001-32-5
• EC Number: 232-598-6
• MDL number: MFCD00131060
• NACRES: NA.32

Properties

• biological source: human plasma
• Quality Level: 300
• description: essentially plasmin(ogen) free
• form: powder
• quality: 35-65% protein (≥90% of protein is clottable).
• mol wt: α-chain 63.5 kDa; β-chain 56 kDa; γ chain 47 kDa (about 4% carbohydrate content); soluble dimer 340 kDa
• concentration: 35-65% protein (biuret)
• technique(s): cell culture | mammalian: suitable
• solubility: 0.9% NaCl: soluble
• UniProt accession no.: P02671; P02675; P02679
• storage temp.: −20°C
• Gene Information: human ... FGA(2243) , FGB(2244) , FGG(2266)

Description

General description

Fibrinogen was the first blood coagulation factor to be identified, and it is a precursor of fibrin protein. This protein is composed of two chains, each of which is composed of three chains- α, β and γ. These three subunits are coded by three genes located in the human chromosomal locus 4q23-q32, and spans 51kb.

Application

Fibrinogen from human plasma has been used for-

• fibrinogen adsorption assay performed on swCNTs (single-walled carbon nanotubes)
• the characterization of fibrinogen structure in the presence of CNT using CD (Circular dichroism) spectroscopy and temperature scan
• the preparation of RBC suspension obtained from venous blood of antecubital vein, to assess RBC aggregability
• fibrinolysis assay performed on mouse embryonic fibroblasts (MEFs)
• the preparation of fibrinogen-labeled gold (Fgn/Au)
• the quantification of in vitro angiogenesis by human microvascular endothelial cells and
• the assessment of fibrin-induced apoptosis and differentiation-regulation in trophoblast obtained from term human placentas.

Biochem/physiol Actions

Fibrinogen is an acute phase protein that is part of the coagulation cascade of proteins. The end result of the cascade is the production of thrombin that converts fibrinogen to fibrin. Thrombin rapidly proteolyses fibrinogen, releasing fibrinopeptide A. The loss of this small peptide is not sufficient to make the resulting fibrin molecule insoluble, but it tends to form complexes with adjacent fibrin and fibrinogen molecules. Thrombin then cleaves a second peptide, fibrinopeptide B, from fibrin and the fibrin monomers formed then polymerize spontaneously to form an insoluble gel. The polymerized fibrin is held together by noncovalent and electrostatic forces and stabilized by the transamidating enzyme, factor XIIIa, that is produced by the action of thrombin on factor XIII. The insoluble fibrin aggregates (clots) and aggregated platelets then block the damaged blood vessel and prevent further bleeding. The amount of fibrinogen in the plasma can serve as a nonspecific indicator of whether or not an inflammatory process is present in the body. Fibrinogen from any mammalian source will be cleaved by thrombin from any mammalian source.

Fibrinogen is an acute-phase inflammatory protein, which plays an essential role in blood clotting. Coronary heart disease is linked to the plasma levels of fibrinogen in Chinese population, and might have potential as a marker and risk factor for the same. A -455 allele of fibrinogen β (FGB) chain is linked with poor survival in Caucasian women with Finnish stroke. Ischemic stroke preceded by hyperfibrinogenemia has poor outcome, which is independent of the baseline fibrinogen concentration.

Specifications

Source material has tested negative for HIV and HBsAg.

Physical form

Lyophilized powder containing sodium citrate and sodium chloride.

Analysis Note

Protein determined by biuret

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves