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F6755

Sigma-Aldrich

Fibrinogen from rat plasma

60-80% protein (≥60% of protein is clottable)

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Synonym(s):
Factor I
CAS Number:
EC Number:
MDL number:
NACRES:
NA.61

biological source

rat plasma

Quality Level

form

powder

quality

60-80% protein (≥60% of protein is clottable)

mol wt

α-chain 63.5 kDa
β-chain 56 kDa
γ chain 47 kDa (about 4% carbohydrate content)
soluble dimer 340 kDa

concentration

60-80% (biuret)

technique(s)

cell culture | mammalian: suitable
immunoblotting: suitable

UniProt accession no.

storage temp.

−20°C

Gene Information

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This Item
F8630F3879F5386
form

powder

form

powder

form

powder

form

powder

mol wt

α-chain 63.5 kDa, β-chain 56 kDa, γ chain 47 kDa (about 4% carbohydrate content), soluble dimer 340 kDa

mol wt

α-chain 63.5 kDa, β-chain 56 kDa, γ chain 47 kDa (about 4% carbohydrate content), soluble dimer 340 kDa

mol wt

α-chain 63.5 kDa, β-chain 56 kDa, γ chain 47 kDa (about 4% carbohydrate content), soluble dimer 340 kDa

mol wt

-

concentration

60-80% (biuret)

concentration

65-85% (biuret)

concentration

50-70% protein (biuret)

concentration

-

technique(s)

cell culture | mammalian: suitable, immunoblotting: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

ELISA: suitable

technique(s)

indirect ELISA: suitable

UniProt accession no.

P02680, P06399, P14480

UniProt accession no.

P02672, P02676, P12799

UniProt accession no.

P02671, P02675, P02679

UniProt accession no.

P02671, P02675, P02679

Application

Fibrinogen from rat plasma has been used:
  • in the preparation of growth factor solutions
  • to apply on discontinuous SDS-polyacrylamide gels for the detection of IgG (immunoglobulins) type antibodies by immunoblot analysis
  • to mix with cell suspension to form a clot and retain the cells for avoiding its dispersion during transplantation

Fibrinogen has been used in studies of haemostatic therapy in surgical and massive trauma patients. These studies have shown that fibrinogen may prove to be more superior in stopping blood loss when compared to using fresh frozen plasma (FFP).

Biochem/physiol Actions

Fibrinogen is an acute phase protein that is part of the coagulation cascade of proteins. The end result of the cascade is the production of thrombin that converts fibrinogen to fibrin. Thrombin rapidly proteolyses fibrinogen, releasing fibrinopeptide A. The loss of this small peptide is not sufficient to make the resulting fibrin molecule insoluble, but it tends to form complexes with adjacent fibrin and fibrinogen molecules. Thrombin then cleaves a second peptide, fibrinopeptide B, from fibrin and the fibrin monomers formed then polymerize spontaneously to form an insoluble gel. The polymerized fibrin is held together by noncovalent and electrostatic forces and stabilized by the transamidating enzyme, factor XIIIa, that is produced by the action of thrombin on factor XIII. The insoluble fibrin aggregates (clots) and aggregated platelets then block the damaged blood vessel and prevent further bleeding. The amount of fibrinogen in the plasma can serve as a nonspecific indicator of whether or not an inflammatory process is present in the body. Fibrinogen from any mammalian source will be cleaved by thrombin from any mammalian source.

Quality

Contains ~12% sodium citrate and ~18% sodium chloride.

Analysis Note

Protein determined by biuret.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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