L-Glutamine, 200 mM solution, tends to precipitate in solution when chilled. After thawing, it will be an opalescent solution. If allowed to settle, there can be some crystalline insolubles at the bottom of the bottle.The product should be warmed to 37°C for complete solubilization. To limit the number of times the product needs to be warmed to 37°C, the product can be divided into working aliquots which are stored at -20°C and used as needed. This will expose the product to the minimal amount of warming cycles.
G2150
L-Glutamine solution
200 mM, Hybri-Max™, sterile-filtered, suitable for hybridoma
Synonym(s):
L-Glutamine for cell culture
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352209
eCl@ss:
32160406
PubChem Substance ID:
NACRES:
NA.75
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SMILES string
N[C@@H](CCC(N)=O)C(O)=O
InChI
1S/C5H10N2O3/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H2,7,8)(H,9,10)/t3-/m0/s1
InChI key
ZDXPYRJPNDTMRX-VKHMYHEASA-N
grade
Hybri-Max™
sterility
sterile-filtered
assay
≥99% (TLC)
form
solution
technique(s)
cell culture | hybridoma: suitable
impurities
endotoxin, tested
color
clear colorless
shipped in
dry ice
storage temp.
−20°C
Quality Level
Gene Information
rat ... Ggt1(116568)
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This Item | |||
|---|---|---|---|
| form solution | form solution | form powder | form powder |
| grade Hybri-Max™ | grade - | grade - | grade - |
| assay ≥99% (TLC) | assay - | assay 99.0-101.0% | assay ≥99% (HPLC) |
| technique(s) cell culture | hybridoma: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - |
| storage temp. −20°C | storage temp. −20°C | storage temp. room temp | storage temp. room temp |
| Quality Level 300 | Quality Level 300, 400 | Quality Level 300 | Quality Level 200 |
General description
L-glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture. L-glutamine is very stable as a dry powder and as a frozen solution. In liquid media or stock solutions, however, L-glutamine degrades relatively rapidly. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use.
Application
The L-Glutamine solution has been used as a supplement in Roswell Park Memorial Institute (RPMI)-1640 medium to culture human leukemia monocytic cell line for intracellular assay and splenocytes for in vitro activation of CD4+ T cells. It has also been used as a supplement to the hybridoma medium used for primary screening for antigen binding and hybridoma cloning.
Preparation Note
Prepared in cell culture grade water.
Legal Information
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
G2150-BULK: + G2150-VAR: + G2150-20ML:
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Articles
Importance and uses of glutamine in hybridoma and mammalian cell culture
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Why is there a precipitate in the 200 mM L-glutamine solution, products G7513 and G2150, after thawing at room temperature?
1 answer-
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Is the molecular weight of L-Glutamine 146.14 or 146.15? The label information changed.
1 answer-
Based on a molecular formula of C5H10N2O3, the molecular weight to seven significant figures is 146.1456. Over the years, this value may have been reported in Sigma-Aldrich documentation or on labels as either 146.15 (by rounding up) or 146.14 (by truncation). The material itself has not changed.
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