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G5154

Glasgow Minimum Essential Medium

With sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

BHK medium, GMEM, Glasgow′s MEM

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500 ML

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¥5,200

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About This Item

MDL number:
MFCD00243118
UNSPSC Code:
12352207
NACRES:
NA.75

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Product Name

Glasgow Minimum Essential Medium, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

L-glutamine: no
phenol red: 0.0213 g/L
NaHCO3: 2.75 g/L
glucose: 4.5 g/L (Dextro)

shipped in

ambient

storage temp.

2-8°C

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This Item
G614851492CG2549
Sigma-Aldrich

Sigma-Aldrich

G5154

Glasgow Minimum Essential Medium

Sigma-Aldrich

Sigma-Aldrich

G6148

Glasgow Minimum Essential Medium

SAFC

SAFC

51492C

Glasgow′s Modified Eagle′s Medium

Glasgow Minimum Essential Medium With sodium bicarbonate and L-glutamine, liquid, sterile-filtered, suitable for cell culture

SAFC

G2549

Glasgow Minimum Essential Medium

sterility

sterile-filtered

sterility

-

sterility

sterile

sterility

sterile-filtered

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable, cell culture | stem cell: suitable

technique(s)

cell culture | mammalian: suitable

form

liquid

form

powder

form

liquid

form

liquid

components

L-glutamine: no
phenol red: 0.0213 g/L
NaHCO3: 2.75 g/L
glucose: 4.5 g/L (Dextro)

components

glucose: 4.5 g/L
phenol red: 0.0213 g/L
L-glutamine: 0.292 g/L
NaHCO3: no

components

L-glutamine: no
sodium pyruvate: 110 mg/L
phenol red: 17 mg/L
NaHCO3: 2750 mg/L

components

phenol red: 0.0213 g/L
L-glutamine: 0.292 g/L
NaHCO3: 2.75 g/L
glucose: 4.5 g/L (Dextro)

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

General description

For use with adherent kidney cell lines such as baby hamster kidney cells (BHK).
Glasgow Minimum Essential Medium has been developed by modifying the Eagle′s BME medium. This medium can be used to study the genetic factors affecting cell competence.

Application

Glasgow Minimum Essential Medium has been used to culture:
  • Hoxb7- green fluorescence protein (GFP) mouse embryonic stem (ES) cell line for the induction of ureteric bud differentiation
  • murine E14 Tg2a embryonic stem (mES) cells
  • transduced Chinese hamster ovary (CHO) cells for the expression and production of Siglec-1-Fc fusion proteins

Preparation Note

Supplement with 0.292 g/L L-glutamine and Tryptose phosphate broth solution (T 8159) at 100 ml/L of medium.

related product

Product No.
Description
Pricing

used together

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

G5154-500ML:
G5154-BULK:
G5154-6X500ML:
G5154-VAR:

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Certificates of Analysis (COA)

Lot/Batch Number
RNBM0085
RNBL4469
RNBL2033
RNBL0511
RNBK9373

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Marco D'Addio et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 35(11), e22017-e22017 (2021-10-27)
Cellular interactions between endothelial cells and macrophages regulate macrophage localization and phenotype, but the mechanisms underlying these interactions are poorly understood. Here we explored the role of sialoglycans on lymphatic endothelial cells (LEC) in interactions with macrophage-expressed Siglec-1 (CD169). Lectin-binding
Marieke Aarts et al.
Genes & development, 31(20), 2085-2098 (2017-11-16)
Expression of the transcription factors OCT4, SOX2, KLF4, and cMYC (OSKM) reprograms somatic cells into induced pluripotent stem cells (iPSCs). Reprogramming is a slow and inefficient process, suggesting the presence of safeguarding mechanisms that counteract cell fate conversion. One such
Cinzia Caprio et al.
Disease models & mechanisms (2021-02-21)
The Ezh2 gene encodes a histone methyltransferase of the Polycomb Repressive Complex 2 that methylates histone H3 lysine 27. In this work we asked whether EZH2 has a role in the development of the pharyngeal apparatus and whether it regulates
Sacha Robert et al.
International journal of molecular sciences, 22(19) (2021-10-14)
Differentiation of pluripotent stem cells to cardiomyocytes is influenced by culture conditions including the extracellular matrices or similar synthetic scaffolds on which they are grown. However, the molecular mechanisms that link the scaffold with differentiation outcomes are not fully known.
Edward C Hsiao et al.
PloS one, 3(7), e2532-e2532 (2008-07-04)
Fluorescent reporters are useful for assaying gene expression in living cells and for identifying and isolating pure cell populations from heterogeneous cultures, including embryonic stem (ES) cells. Multiple fluorophores and genetic selection markers exist; however, a system for creating reporter
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