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G8540

Sigma-Aldrich

L-Glutamine

meets USP testing specifications, suitable for cell culture, 99.0-101.0%, from non-animal source

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Synonym(s):
(S)-2,5-Diamino-5-oxopentanoic acid, L-Glutamic acid 5-amide, Levoglutamide
Linear Formula:
H2NCOCH2CH2CH(NH2)CO2H
CAS Number:
Molecular Weight:
146.14
Beilstein:
1723797
EC Number:
MDL number:
eCl@ss:
32160406
PubChem Substance ID:
NACRES:
NA.26

biological source

non-animal source

Quality Level

Agency

meets USP testing specifications

Assay

99.0-101.0%

form

powder

optical activity

[α]20/D 31.5 to 33.0°, c = 10 in 2 M HCl
[α]20/D 6.3 to 7.3°, c = 4 in water

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

color

white

mp

185 °C (dec.) (lit.)

solubility

H2O: 25 mg/mL

cation traces

As: ≤1 ppm
Fe: ≤10 ppm
NH4+: ≤0.10%

application(s)

agriculture

functional group

amide

storage temp.

room temp

SMILES string

N[C@@H](CCC(N)=O)C(O)=O

InChI

1S/C5H10N2O3/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H2,7,8)(H,9,10)/t3-/m0/s1

InChI key

ZDXPYRJPNDTMRX-VKHMYHEASA-N

Gene Information

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1 of 4

This Item
G6392G5792G3126
vibrant-m

G8540

L-Glutamine

vibrant-m

G6392

L-Glutamine

vibrant-m

G5792

L-Glutamine

vibrant-m

G3126

L-Glutamine

assay

99.0-101.0%

assay

-

assay

≥99%

assay

≥99% (HPLC)

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

functional group

amide

functional group

-

functional group

-

functional group

amide

storage temp.

room temp

storage temp.

-

storage temp.

-

storage temp.

room temp

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, heavy metals, residual solvents, tested

impurities

≤0.2% free ammonia., ~0.5% Glutamic acid

General description

L-Glutamine is the most abundant amino acid in mammalian blood and tissues. It is vital for biochemical and cell culture research. It supports cell growth, metabolism, and protein synthesis. In cell culture, it ensures cell viability, proliferation, and antibody production. In biochemical studies, L-Glutamine aids in metabolic pathways, biomolecule synthesis, and cellular processes.

Application

L-Glutamine amino acid has been used:

  • as a component of the serum-free medium to culture mice cochlear organotypic cells.
  • as a supplement in Dulbecco′s modified Eagle′s medium-nutrient mixture F-12 (DMEM/F-12) medium for culturing mice seminiferous tubule segments.
  • as a supplement in Roswell Park Memorial Institute (RPMI) 1640 medium to culture human monocytic cell line.

Biochem/physiol Actions

L-Glutamine or Glutamine powder serves as a precursor for the synthesis of peptides, proteins, nucleotides, purine, pyrimidine, amino sugars, and nucleic acids. It plays an important role in promoting and maintaining cellular functions. Many cells and tissues like the intestine, kidneys, neurons in the central nervous system (CNS), liver, and pancreatic β-cells utilize glutamine at higher levels for their functioning. Glutamine regulates acid-base homeostasis and catabolic signaling. Glutamine plays a vital role in nitrogen transport and maintains the cellular redox state.Glutamine provides the necessary nitrogen, carbon and the reducing equivalents required for cell growth and division during cell culture. L-glutamic acid aids the incorporation of NH4+ into biomolecules.
L-Glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture. L-Glutamine is very stable as a dry powder and as a frozen solution. In liquid media or stock solutions, however, L-glutamine degrades relatively rapidly. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Glutamine and glutamate?their central role in cell metabolism and function
Newsholme P, et al.
Cell Biochemistry and Function, 21(1) (2003)
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Chromatograms

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