H4034
HEPES
BioPerformance Certified, ≥99.5% (titration), suitable for cell culture
Recommended Products
grade
BioPerformance Certified
Quality Level
Assay
≥99.5% (titration)
form
crystalline powder
storage condition
dry at room temperature
technique(s)
cell culture | mammalian: suitable
competitive inhibition ELISA: suitable
impurities
endotoxin and total aerobic microbial count, tested
color
white
useful pH range
6.8-8.2
pKa (25 °C)
7.5
cation traces
Fe: ≤5 ppm
absorption
≤0.05 at 260 in H2O at 0.1 M
≤0.05 at 280 in H2O at 0.1 M
suitability
suitable for Western blot
suitable for component for culture media
application(s)
clinical research
diagnostic assay manufacturing
general analytical
life science and biopharma
foreign activity
DNase, RNase, NICKase, protease, none detected
SMILES string
OCCN1CCN(CC1)CCS(O)(=O)=O
InChI
1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
InChI key
JKMHFZQWWAIEOD-UHFFFAOYSA-N
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General description
HEPES also proves advantageous in various biological and biochemical processes. Its ampholytic nature makes it suitable as a separator for creating pH gradients in isoelectric focusing, a technique often employed in protein separation and analysis. Moreover, HEPES exhibits minimal interference with DNA-restriction enzyme reactions compared to buffers with fewer substituted amine groups, such as Tris. This makes it a preferred choice for applications involving DNA manipulation and analysis. Beyond these specific applications, HEPES is valuable in numerous other biological and biochemical processes, including immunoprecipitation, cell lysis, and live cell imaging. Its versatility, coupled with exceptional pH buffering capacity and minimal interaction with other molecules, establishes HEPES as an indispensable tool across diverse research domains.
Application
- To supplement Dulbecco′s modified Eagle′s medium to culture and maintain cell lines
- As a component of platelet suspension buffer
- To supplement Hank′s basic salt solution, which is used to wash pancreatic tissue
- As a component of wash buffer and blocking buffer in the purification and quantification of protein with enzyme-linked immunosorbent (ELISA) assay
- For the adjustment and maintenance of pH of biological solutions
- As a component of Hank′s balanced salt solution (HBSS) and dissociation medium to study neuronal development
- For homogenization of tissue and in the preparation of cytosolic and nuclear extract from cells
- As a component of keratinocyte and fibroblast culture medium
Features and Benefits
- Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
- Highly soluble in water with a useful pH range of 6.8 - 8.2 and pKa of 7.5 at 25 °C
- Negligible metal ion binding
- Less toxic to cells than other buffers such as Tris and phosphate
- Stable in a wide pH range
- Tested for Endotoxins and Total Aerobic Microbial Count
- Free from DNase, NICKase, RNase, Endonuclease, Exonuclease and Protease
Other Notes
also commonly purchased with this product
comparable product
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
JAN Code
H4034-BULK-PC:
H4034-1KG:4548173199320
H4034-BULK:
H4034-25G:4548173199337
H4034-1KG-PB:
H4034-RSAMPLE:
H4034-500G-PB:
H4034-25KG-DP:
H4034-50KG:4548173199368
H4034-25KG:4548173199344
H4034-VAR:
H4034-6X500G:4548173199375
H4034-EW:
H4034-500G:4548173199351
2508010:
H4034-100G:4548173199313
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Protocols
Formaldehyde cross-linking protocol detects specific protein interactions with confidence.
Techniques for protein antigen molecular weight determination, protein interactions, enzymatic activity, and post-translational modifications.
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