H4641

Hanks′ Balanced Salt Solution 10x

Without calcium chloride, magnesium sulfate and sodium bicarbonate, 10 ×, liquid, sterile-filtered, suitable for cell culture

• Synonym(s): Hanks’ Balanced Salt solution, HBSS
• NACRES: NA.75

Properties

• Quality Level: 400
• sterility: sterile-filtered
• form: liquid
• concentration: 10 ×
• technique(s): cell culture | mammalian: suitable
• components: phenol red: yes
• shipped in: ambient

Description

General description

Balanced salt solution (BSS) is crucial in cell culture as it serves as an irrigating, transporting, and diluting agent to maintain the intra- and extracellular osmotic balance and also provides energy for cell metabolism. It provides water and bulk inorganic ions to the cells to maintain the normal cell metabolism. BSS plays a role in the buffering system to maintain the medium within the physiological pH range (7.2-7.6).

Application

Hanks′ Balanced Salt Solution 10x has been used:

• as a diluting agent during the Chinese hamster ovary cell line detachment from growth medium
• to wash the biopsy specimens of lamina propria lymphocytes (LPLs)
• as a wash solution to wash the fish cell line during removal from growth medium

Reconstitution

Supplement with 0.35 g/L sodium bicarbonate at 1X.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable