H4791

Bone Morphogenetic Protein 2 human

BMP-2, recombinant, expressed in HEK 293 cells, HumanKine, suitable for cell culture

• Synonym(s): BMP-2
• MDL number: MFCD03097877
• NACRES: NA.77

Properties

• biological source: human
• Quality Level: 100
• recombinant: expressed in HEK 293 cells
• form: lyophilized powder
• potency: ≤60 ng/mL EC₅₀
• quality: endotoxin tested
• mol wt: dimer 30-38 kDa (glycosylated)
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1 EU/μg Endotoxin level
• UniProt accession no.: P12643
• storage temp.: −20°C
• Gene Information: human ... BMP2(650)

Description

General description

Bone Morphogenetic Protein 2 is a member of the TGF-β superfamily of cytokines that affect bone and cartilage formation. It is important for skeletal development during embryogenesis. BMP-2 induces chondrocyte formation, osteoblast differentiation, and is involved in embryo dorsal-ventral patterning and organogenesis.

Biochem/physiol Actions

It has been reported that Bone Morphogenetic Protein 2 (BMP-2) inhibits estradiol induced proliferation of human breast cancer cells. BMP-2 signaling mediates apoptosis by activation of the TAK1-p38 kinase pathway that is negatively regulated by Smad6. Cellular responses to BMP-2 are mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors, which play significant roles in BMP binding and signaling. One BMP type II receptor and two BMP type I receptors have been identified. Both BMP type I receptors bind BMP-2 with high-affinity in the absence of BMP receptor type II.

Physical form

Lyophilized from a 0.2 μm filtered solution of 2x PBS + 6% Ethanol.

Preparation Note

This Bone Morphogenetic Protein 2 (BMP-2) is produced from a DNA sequence encoding the human BMP-2 protein, expressed in HEK 293 cells. It is a glycosylated homodimer linked by a single disulfide bond with an apparent molecular mass of 30-38 kDa.

Analysis Note

The specific activity was determined by its ability to induce alkaline phosphatase production in a dose response to BMP-2 in the ATDC-5 cell line (mouse chondrogenic cell line).

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable