Recommended Products
Quality Level
Assay
≥99.0% (titration)
form
powder
storage condition
dry at room temperature
technique(s)
HPLC: suitable
color
white
pH
10-12 (1 M in water)
useful pH range
6.8-8.2
pKa (25 °C)
7.5
solubility
water: 0.1 M, clear, colorless
suitability
suitable for mass spectrometry
application(s)
diagnostic assay manufacturing
general analytical (
)
life science and biopharma
metabolomics
storage temp.
room temp
SMILES string
[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O
InChI
1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1
InChI key
RDZTWEVXRGYCFV-UHFFFAOYSA-M
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General description
Widely utilized in cell biology and biochemical research, HEPES proves its versatility in protein purification and extraction protocols, owing to its stability across a broad pH range and minimal metal ion binding. Additionally, HEPES proves valuable in various biological and biochemical processes, including immunoprecipitation, cell lysis, and live cell imaging, making it a versatile tool in diverse research applications.
Application
- Most studies with metal ions
- Substitute for Tris and phosphate in studies with metal ions
- Grinding buffer in plant studies
- Running buffer in gel electrophoresis
- Buffer in electroporation studies
- Bradford or bicinchoninic acid (BCA) assays
- Extraction buffer to prevent damage of proteins in red blood cells
It has been used:
- as a buffer component in the preparation of a low sucrose buffer
- as a buffer component in a modified Hanks′ solution, a pseudo-physiological solutions
Features and Benefits
- Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
- Highly soluble in water
- Negligible metal ion binding
- Less toxic to cells than other buffers such as Tris and phosphate
- Stable in a wide pH range
Physical form
Other Notes
comparable product
related product
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
JAN Code
H7006-RSAMPLE:
H7006-10G:
H7006-VAR:
H7006-25KG:
H7006-25G:
H7006-6KG:
H7006-PM:
2008015:
H7006-500G:
H7006-5KG:
2540000:
2008015-250G:
H7006-2.5KG:
H7006-BULK-PC:
H7006-1KG:
H7006-BULK:
H7006-250G:
H7006-100G:
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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