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Merck

I7403

L-Isoleucine

98.5-101.0%, suitable for cell culture, BioXtra, non-animal source

Synonym(s):

(2S,3S)-2-Amino-3-methylpentanoic acid

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¥15,235

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¥32,615

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¥176,550

¥2,849

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About This Item

Linear Formula:
C2H5CH(CH3)CH(NH2)CO2H
CAS Number:
Molecular Weight:
131.17
NACRES:
NA.26
PubChem Substance ID:
eCl@ss:
32160406
UNSPSC Code:
12352209
EC Number:
200-798-2
MDL number:
Beilstein/REAXYS Number:
1721792

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Product Name

L-Isoleucine, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 98.5-101.0%

InChI

1S/C6H13NO2/c1-3-4(2)5(7)6(8)9/h4-5H,3,7H2,1-2H3,(H,8,9)/t4-,5-/m0/s1

SMILES string

CC[C@H](C)[C@H](N)C(O)=O

InChI key

AGPKZVBTJJNPAG-WHFBIAKZSA-N

biological source

non-animal source

agency

USP/NF
meets EP testing specifications
meets JP testing specifications
meets USP testing specifications

product line

BioXtra

assay

98.5-101.0%

form

powder

quality

meets EP, JP, USP testing specifications

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

color

white

mp

288 °C (dec.) (lit.)

solubility

1 M HCl: 50 mg/mL

application(s)

peptide synthesis
pharmaceutical (small molecule)

Quality Level

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1 of 4

This Item
1349502I2752I5281
form

powder

form

-

form

powder

form

crystalline powder

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

technique(s)

cell culture | mammalian: suitable

assay

98.5-101.0%

assay

-

assay

≥98% (HPLC)

assay

≥98.5%

product line

BioXtra

product line

-

product line

-

product line

-

impurities

endotoxin, tested

impurities

-

impurities

-

impurities

endotoxin, heavy metals, residual solvents, tested

solubility

1 M HCl: 50 mg/mL

solubility

-

solubility

1 M NH4OH: 50 mg/mL

solubility

1 M HCl: 50 mg/mL

Application

L-Isoleucine has been used as a supplement of maintenance media consisting of DMEM/F-12 for the culture of primary hepatocytes. It has also been used as a supplement for serial culture of adult murine keratinocytes.[1][2]

Biochem/physiol Actions

L-Isoleucine is an essential amino acid that is synthesized from threonine.[3][4] It is a branched-chain hydrophobic amino acid.[5][6] L-isoleucine is an isomer of L-leucine.[7]

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Biochemistry (5th Edition) null
Inhibitory effect of aroma on the bitterness of branched-chain amino acid solutions.
Mukai J
Chemical & Pharmaceutical Bulletin, 55(11), 1581-1584 (2007)
Recapitulation of metabolic defects in a model of propionic acidemia using patient-derived primary hepatocytes.
Chapman KA
Molecular Genetics and Metabolism, 117(3), 355-362 (2016)
Conversion of ammonia or urea into essential amino acids,L-leucine,L-valine, andL-isoleucine using artificial cells containing an immobilized multienzyme system and dextran-NAD+
Kang Fu Gu
Applied Biochemistry and Biotechnology, 26 (1990)
John W Hill
Chemistry for Changing Times (2016)

Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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