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M1302

M-MLV Reverse Transcriptase

Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis

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Synonym(s):

Moloney Murine Leukemia Virus Reverse Transcriptase

CAS Number:

9068-38-6

MDL number:

MFCD00283754

NACRES:

NA.55

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HIV Reverse Transcriptase, Recombinant, E. coli

recombinant

expressed in E. coli

Quality Level

200

form

liquid

usage

sufficient for 200 reactions
sufficient for 250 reactions

feature

dNTPs included: no
hotstart: no

concentration

200 units/μL

technique(s)

RT-PCR: suitable

color

colorless

input

purified RNA

shipped in

wet ice

storage temp.

−20°C

Related Categories

PCR Reagents & Kits

General description

Moloney murine leukemia virus (M-MLV ) reverse transcriptase enzyme is isolated from E. coli expressing a portion of the pol gene of M-MLV on a plasmid. MoMLV RT is made up of 671 amino acid residues.[1] It is a DNA polymerase that uses single-stranded RNA, DNA, or an RNA-DNA hybrid (using a primer) to synthesize a complementary DNA strand.

Application

M-MLV Reverse Transcriptase has been used:

for the preparation of cDNA libraries or for first strand cDNA synthesis[2][3]
for use in a 2-step RT-PCR assay[4][5][6]
in quantitative realtime-polymerase chain reaction (RT-qPCR)[5]
in reverse transcription[6]

Features and Benefits

Thermostable reverse transcriptase active at 37 °C.
Can be used to generate first strand cDNA of up to 7 kb.

Packaging

Supplied with 10× M-MLV reverse transcriptase buffer containing DTT.

Unit Definition

One unit incorporates 1 nmol of TTP into acid precipitable material in 10 min. at 37 °C using poly(A):oligo dT as a template:primer.

Preparation Note

The enzyme is purified from Escherichia coli expressing the pol gene of M-MLV on a plasmid.

Legal Information

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.