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M5755

Sigma-Aldrich

MOPS-EDTA-Sodium Acetate (MESA)

powder for RNA electrophoresis buffers

Synonym(s):

MESA Buffer

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1 L
¥64,500

¥64,500

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1 L
¥64,500

About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

¥64,500

Check Cart for Availability
Request a Bulk Order

grade

Molecular Biology

Quality Level

200

product line

BioReagent

form

powder

technique(s)

electrophoresis: suitable

pH

6.8-7.2(reconstituted per directions)

solubility

H2O: soluble, faintly yellow

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

storage temp.

room temp

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This Item
T8280T7527PPB008
MOPS-EDTA-Sodium Acetate (MESA) powder for RNA electrophoresis buffers

M5755

MOPS-EDTA-Sodium Acetate (MESA)

Tris Acetate-EDTA buffer 10× concentrate, BioReagent, Molecular Biology, DNase and RNase, none detected, powder blend, suitable for electrophoresis

T8280

Tris Acetate-EDTA buffer

Tris-Borate-EDTA buffer BioReagent, Molecular Biology, 5x concentrate, DNase and RNase, none detected, powder blend, suitable for electrophoresis

T7527

Tris-Borate-EDTA buffer

Tris Acetate-EDTA buffer pH 8.3, pHast Pack™, powder

PPB008

Tris Acetate-EDTA buffer

grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

grade

-

technique(s)

electrophoresis: suitable

technique(s)

-

technique(s)

-

technique(s)

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

-

product line

BioReagent

product line

BioReagent

product line

BioReagent

product line

pHast Pack™

form

powder

form

powder blend

form

powder blend

form

powder

storage temp.

room temp

storage temp.

-

storage temp.

-

storage temp.

-

General description

MOPS – EDTA – Sodium Acetate (MESA) is the most commonly used running buffer for RNA electrophoresis prior to Northern blotting procedures. Applied voltage of 5 V/cm is recommended for maximum resolution.

Application

Suitable for use when making formaldehyde-agarose gels and associated running buffer for RNA electrophoresis.

Preparation Note

1x MESA buffer contains:
  • 40 mM MOPS
  • 10 mM sodium acetate
  • 1 mM EDTA (pH 8.3)

Reconstitution

Dissolve entire contents of bottle in a final volume of 1L molecular biology grade water. This produces a 10x MESA stock solution that can be further diluted as needed.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PRTR

Class I Designated Chemical Substances

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

M5755-1L:
M5755-BULK:
M5755-VAR:

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Certificates of Analysis (COA)

Lot/Batch Number
0000383887
0000257463
0000257463
0000344083
0000383887

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