Merck
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N3520

Sigma-Aldrich

Nutrient Mixture F-12 Ham

Kaighn′s Modification, with L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

All Photos(1)
Synonym(s):
Ham’s F-12
MDL number:
MFCD00217417
NACRES:
NA.75

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sterility

sterile

form

powder

technique(s)

cell culture | mammalian: suitable

components

NaHCO3: no
sodium pyruvate: 0.22 g/L
HEPES: no
L-glutamine: yes
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

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General description

The nutrient mixture Ham′s F-12 is developed by Ham and it has higher levels of amino acids, vitamins and trace elements. Putrescine and linoleic acid are added to the formulation. Nutrient medium was originally designed for the serum-free growth of Chinese hamster ovary and lung cells. When used with whole or dialyzed serum or in combination with hormones and transferrin, it is widely used to grow mammalian and hybridoma cells.

Application

Nutrient Mixture F-12 Ham is used for the following applications:
  • HeLa (human cervix adenocarcinoma cells) were grown in this medium (nutrient mixture F-12 Ham) supplemented with 10% heat-inactivated fetal calf serum[1]
  • Used in cell culture for different cells during maintenance and incubation (i.e. A549 cell line was maintained as a monolayer in nutrient mixture F-12 HAM Medium along with other components)[2][3]
  • Used in media for the propagation of Cowdria ruminantium[4]
  • Used during preparation of primary mixed glial cultures[5]

Kaighn′s modification of Ham′s F-12 and Coon′s F-12 has increased concentrations of amino acids and pyruvate, as well as modified salts (Konigsbergs). This medium was designed to support the growth of differentiated rat and chicken cells, and primary human liver cells.

Quantity

Formulated to contain 11.1 grams of powder per liter of medium.

Reconstitution

Supplement with 2.5 g/L sodium bicarbonate.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317

Hazard Classifications

Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

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ISHL Notified Names

Substances Subject to be Notified Names

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

Product Information Sheet - N3520

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