Merck
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NA0160

Sigma-Aldrich

GenElute HP Plasmid Miniprep Kit

greener alternative

sufficient for 350 preparations

Synonym(s):
GenElute HP Plasmid Kit, HP miniprep kit, fast miniprep kit, fast plasmid isolation kit, plasmid extraction kit, plasmid isolation kit, plasmid purification kit, ultrafast plasmid isolation kit, Gen Elute
NACRES:
NA.52

usage

sufficient for 350 preparations

Quality Level

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative category

storage temp.

15-25°C

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General description

An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the plasmid DNA onto silica in the presence of high salts. Contaminants are then removed by a vacuum or spin wash steps. Finally, the bound plasmid DNA is eluted in water or Tris-EDTA buffer.

The GenElute HP Plasmid Miniprep Kit offers an ultrafast and efficient solution for plasmid preparation from E. coli cultures. This kit combines silica-based membrane technology and the convenience of spin or vacuum format,up to 25 μg of high copy plasmid DNA can be recovered from 1-5 mL of E. coli culture in less than 30 minutes. Note that actual yield and optimum volume of culture to use depend on the plasmid and the culture medium.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Application

The recovered plasmid DNA is ready for immediate use in applications such as restriction enzyme digestion, cloning, PCR, transcription, and sequencing.

Features and Benefits

  • Purified plasmid DNA in less than 30 minutes
  • Up to 25 μg of high-copy plasmid DNA
  • Flexibility of a vacuum or spin format
  • No phenol/chloroform extraction or alcohol precipitation required

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3

Target Organs

Central nervous system

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

SDS

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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