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NA0410

Sigma-Aldrich

GenElute HP Endotoxin-Free Plasmid Maxiprep Kit

greener alternative

sufficient for 25 preparations

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Synonym(s):
Gen Elute, GenElute Endotoxin-Free Plasmid Kit
NACRES:
NA.52

usage

sufficient for 25 preparations

Quality Level

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

technique(s)

DNA extraction: suitable

greener alternative category

storage temp.

15-25°C

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This Item
NA0400NA0600NA0400S
usage

sufficient for 25 preparations

usage

sufficient for 10 preparations

usage

 kit sufficient for 5 preparations

usage

sufficient for 4 preparations

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

NA0400

NA0400S, NA0410

-

NA0400, NA0410

greener alternative category

Aligned,

greener alternative category

Aligned,

greener alternative category

, Aligned

greener alternative category

Aligned,

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

-

General description

The GenElute HP Endotoxin-Free Plasmid Maxiprep Kit offers a simple and rapid method for isolating endotoxin-free plasmid DNA from recombinant E. coli cultures. The kit uses a vacuum format with a filter column for the rapid clearing of the bacterial lysate and a silica column for capturing plasmid DNA. A proprietary solution binds plasmid DNA to the binding column while preventing endotoxins from adsorbing. The technology allows for the user to consistently achieve levels of endotoxin that are less than 0.1 endotoxin units per μg of plasmid DNA.

High-quality, endotoxin-free DNA is ready for immediate use for the most demanding applications including transfection with endotoxin-sensitive cells.

Application

GenElute HP Endotoxin-Free Plasmid Maxiprep Kit has been used in the isolation of plasmid from swine liver and from competent Escherichia coli cells.

Features and Benefits

  • Up to 1.2 mg of high-copy plasmid DNA with endotoxin levels of ≤0.1 EU/μg
  • Only 35 minutes from pelleted cells to purified plasmid
  • Convenient vacuum format

Principle

An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis. The lysate is clarified by filtration followed by the addition of a binding solution that has been optimized for endotoxin-free plasmid preparations. The plasmid DNA is then captured on silica, while endotoxins are prevented from adsorbing to the membrane. Two wash steps remove contaminants. Finally, the bound DNA is eluted in endotoxin-free water. The recovered plasmid DNA is predominately in its supercoiled form. Genomic DNA and RNA are below detectable levels by ethidium bromide stained agarose gel electrophoresis.

Other Notes

For additional information, please see www.sigma-aldrich.com/genelutehp.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Central nervous system

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

77.0 °F - closed cup

Flash Point(C)

25 °C - closed cup


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Please refer to KIT Component information

PRTR

Please refer to KIT Component information

FSL

Please refer to KIT Component information

ISHL Indicated Name

Please refer to KIT Component information

ISHL Notified Names

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Cartagena Act

Please refer to KIT Component information

JAN Code

キットコンポーネントの情報を参照してください


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Successful liver-directed gene delivery by ERCP-guided hydrodynamic injection (with videos)
Kumbhari V, et al.
Gastrointestinal Endoscopy, 88(4), 755-763 (2018)
Towards a metalloprotease-DNA vaccine against piscine cryptobiosis caused by Cryptobia salmositica
Tan CW, et al.
Parasitology Research, 102(2), 265-275 (2008)
Keith Hansen et al.
Journal of visualized experiments : JoVE, (64)(64), e3304-e3304 (2012-06-27)
Genome editing is a powerful technique that can be used to elucidate gene function and the genetic basis of disease. Traditional gene editing methods such as chemical-based mutagenesis or random integration of DNA sequences confer indiscriminate genetic changes in an
Ming-Wei Chen et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(36), 13538-13543 (2008-09-04)
H5N1 influenza viruses have spread extensively among wild birds and domestic poultry. Cross-species transmission of these viruses to humans has been documented in over 380 cases, with a mortality rate of approximately 60%. There is great concern that a H5N1
João M Furtado et al.
Investigative ophthalmology & visual science, 53(11), 6856-6862 (2012-09-07)
Toxoplasma gondii, the parasite responsible for ocular toxoplasmosis, accesses the retina from the bloodstream. We investigated the dendritic cell as a potential taxi for T. gondii tachyzoites moving across the human retinal endothelium, and examined the participation of adhesion molecules

Related Content

Perform rapid isolation of endotoxin-free plasmid DNA using a streamlined procedure that can be completed in as fast as 35 minutes. The GenElute HP Endotoxin-Free Plasmid Maxiprep Kit delivers high plasmid recoveries with levels of endotoxin that are consistently below the industry standard of 0.1 EU/µg.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service