Merck
All Photos(1)

NA0800

Sigma-Aldrich

GenElute HP Select Plasmid Gigaprep Kit

greener alternative

sufficient for 5 preparations

Synonym(s):
HP gigaprep kit, endo free gigaprep kit, endotoxin free giga kit, endotoxin free plasmid kit, fast gigaprep kit, fast plasmid isolation, plasmid extraction kit, plasmid isolation kit, plasmid purification kit, ultrafast plasmid isolation, Gen Elute, GenElute HP Plasmid Kit
NACRES:
NA.52

usage

sufficient for 5 preparations

Quality Level

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative category

storage temp.

15-25°C

General description

The GenElute HP Select Plasmid Gigaprep Kit offers a simple, rapid, and cost effective method for isolating endotoxin-free plasmid DNA from recombinant E. coli cultures. Up to 15 mg of high copy plasmid DNA with ≤0.1 endotoxin unit/μg plasmid DNA can be recovered from E. coli cultures in 1.2-2.5 L Luria Broth (LB) medium or 600 mL-1.2 L of Terrific Broth (TB) medium in less than 2 hours after harvesting the cells. Note that the actual yield depends on the strain, the plasmid, and the culture medium used.

An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis. The lysate is cleared by filtration followed by the addition of a binding solution optimized for endotoxin-free plasmid preparations. The plasmid DNA is then captured onto a silica membrane in the presence of high salts while endotoxins are prevented from adsorbing to the membrane. Contaminants are removed with three wash steps. Finally, the bound DNA is eluted in endotoxin-free water.

The DNA is ready for immediate use in applications such as restriction digest, cloning, sequencing, PCR, and transfection of endotoxin-sensitive cell lines.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Application

GenElute® HP Select Plasmid Gigaprep Kit has been used to purify plasmids.
High-quality, endotoxin-free DNA is ready for immediate use for the most demanding applications including transfection with endotoxin-sensitive cells and gene therapy research.

Features and Benefits

  • Up to 15 mg with endotoxin levels of ≤ 0.1 EU/μg
  • Only 90 minutes from pelleted cells to purified plasmid
  • Vacuum format with no ethanol precipitation
  • Can purify low-, medium-, and high-copy plasmid DNA

Other Notes

For additional information, please see www.sigma-aldrich.com/genelutehp.

Legal Information

Gabitril is a registered trademark of Abbott Laboratories Corp.
GenElute is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.
Description
SDS

  • G3671GenElute HP Pro Gigaprep Binding Column

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT RE 1 Inhalation - STOT SE 3

Target Organs

Central nervous system, Lungs

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

77.0 °F - closed cup

Flash Point(C)

25 °C - closed cup

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service