Merck
  • P3803
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P3803

Sigma-Aldrich

Phenol:Chloroform:Isoamyl Alcohol 25:24:1, Saturated with 10mM Tris, pH 8.0, 1mM EDTA

for molecular biology

All Photos(1)
Synonym(s):
DNA extraction
NACRES:
NA.52

Recommended Products

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Sigma-Aldrich

Sigma-Aldrich

P2069

Phenol:Chloroform:Isoamyl Alcohol 25:24:1 Saturated with 10 mM Tris, pH 8.0, 1 mM EDTA

Supplied with Equilibration buffer, for molecular biology

Phenol – chloroform – isoamyl alcohol mixture BioUltra, for molecular biology, 25:24:1

Sigma-Aldrich

77617

Phenol – chloroform – isoamyl alcohol mixture

BioUltra, for molecular biology, 25:24:1

Millipore

Millipore

516726

Phenol/Chloroform/Isoamyl Alcohol, 25:24:1 (v/v), Molecular Biology Grade - Calbiochem

A 25:24:1 (v/v) mixture of phenol, chloroform and isoamyl alcohol, pH 6.7. Suitable for DNA and RNA applications.

Sigma-Aldrich

Sigma-Aldrich

C0549

Chloroform:Isoamyl alcohol 24:1

suitable for nucleic acid purification

Sigma-Aldrich

Sigma-Aldrich

P1944

Phenol:Chloroform:Iso-amyl alcohol (125:24:1)

for molecular biology

Phenol solution Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, BioReagent, for molecular biology

Sigma-Aldrich

P4557

Phenol solution

Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, BioReagent, for molecular biology

Chloroform – isoamyl alcohol mixture BioUltra, for molecular biology, 24:1, ≥99.5% (chloroform + isoamyl alcohol, GC)

Supelco

25666

Chloroform – isoamyl alcohol mixture

BioUltra, for molecular biology, 24:1, ≥99.5% (chloroform + isoamyl alcohol, GC)

Phenol solution Saturated with 0.1 M citrate buffer, pH 4.3 ± 0.2, BioReagent, for molecular biology

Sigma-Aldrich

P4682

Phenol solution

Saturated with 0.1 M citrate buffer, pH 4.3 ± 0.2, BioReagent, for molecular biology

Phenol – chloroform – isoamyl alcohol mixture BioUltra, for molecular biology, 125:24:1

Supelco

77619

Phenol – chloroform – isoamyl alcohol mixture

BioUltra, for molecular biology, 125:24:1

Phenol for molecular biology

Sigma-Aldrich

P1037

Phenol

for molecular biology

grade

for molecular biology

Quality Level

200

pH

6.5-6.9(pH of phenolic phase)

application(s)

agriculture

storage temp.

2-8°C

Application

Removal of proteins from nucleic acids can be achieved by extraction with phenol:chloroform: isoamyl alcohol solutions. For crude mixtures of nucleic acids, digestion with a broad range proteolytic enzyme prior to the phenol:chloroform extraction may be beneficial. Although proteins are efficiently denatured by phenol, RNase activity is not completely inhibited. Therefore, a small amount of isoamyl alcohol is added to further ensure the deactivation of RNase activity. Please see Product Information Sheet for the protocol for removal of proteins from nucleic acids.

Packaging

100, 400 mL in glass bottle

Warning

If phenol solutions come in contact with skin, burns will occur quickly. It is, therefore, required that the user wear appropriate laboratory clothing including gloves and protective eyewear. In case of direct skin contact, rinse extensively with water and seek medical attention if necessary.

Other Notes

This product has a shelf life of two years.

Recommended products

For DNA extraction, the pH of the phenol phase can be adjusted to 8.0 by equilibration with tris buffer. Sigma offers product P2069 specifically for DNA extractions. This product includes equilibration buffer for the convenience of adjusting the pH.

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2 - STOT SE 3

Target Organs

Central nervous system

Storage Class Code

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Poisonous_And_Deleterious

Deleterious substance

PRTR

Class I Designated Chemical Substances

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

P3803-100ML:4548173206424
P3803-400ML:4548173206431

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Product Information Sheet

Product Information Sheet - P3803

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