P2069 is P3803 supplied with an equilibration buffer. Equilibration buffer B5658-1EA is required for DNA extraction.
P3803
Phenol:Chloroform:Isoamyl Alcohol 25:24:1, Saturated with 10mM Tris, pH 8.0, 1mM EDTA
Molecular Biology
Synonym(s):
DNA extraction
Sign In to View Organizational & Contract Pricing
Select a Size
P3803-100ML
¥9,975
P3803-400ML
¥27,650
¥9,975
List Price¥13,300Save 25%Check Cart for Availability
About This Item
UNSPSC Code:
12190000
NACRES:
NA.52
Grade:
Molecular Biology
Assay:
≥99.0%
Application(s):
agriculture
Skip To
grade
Molecular Biology
Quality Level
Assay
≥99.0%
form
liquid
pH
6.5-6.9(pH of phenolic phase)
application(s)
agriculture
storage temp.
2-8°C
1 of 4
This Item | P2069 | P1944 | P4682 |
|---|---|---|---|
| application(s) agriculture | application(s) agriculture | application(s) life science and biopharma | application(s) - |
| grade for molecular biology | grade for molecular biology | grade for molecular biology | grade for molecular biology |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| assay ≥99.0% | assay ≥99.0% | assay ≥99.0% | assay - |
| form liquid | form liquid | form liquid | form liquid |
| pH 6.5-6.9(pH of phenolic phase) | pH 6.5-6.9(pH of phenolic phase), 7.8-8.2(pH of phenolic phase after the addition of Equilibration Buffer) | pH 4.3-4.7 | pH 4.1-4.5 (25 °C) |
Application
Removal of proteins from nucleic acids can be achieved by extraction with phenol:chloroform: isoamyl alcohol solutions.[1][2][3][4] For crude mixtures of nucleic acids, digestion with a broad range proteolytic enzyme prior to the phenol:chloroform extraction may be beneficial. Although proteins are efficiently denatured by phenol, RNase activity is not completely inhibited. Therefore, a small amount of isoamyl alcohol is added to further ensure the deactivation of RNase activity. Please see Product Information Sheet for the protocol for removal of proteins from nucleic acids.
Other Notes
For DNA extraction, the pH of the phenol phase can be adjusted to 8.0 by equilibration with tris buffer. Sigma offers product P2069 specifically for DNA extractions. This product includes equilibration buffer for the convenience of adjusting the pH.
This product has a shelf life of two years.
Disclaimer
If phenol solutions come in contact with skin, burns will occur quickly. It is, therefore, required that the user wear appropriate laboratory clothing including gloves and protective eyewear. In case of direct skin contact, rinse extensively with water and seek medical attention if necessary.
related product
Product No.
Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2 - STOT SE 3
Target Organs
Central nervous system, Liver,Kidney, Nervous system,Kidney,Liver,Skin
Storage Class Code
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
PDSCL
Deleterious substance
PRTR
Class I Designated Chemical Substances
ISHL Indicated Name
Substances Subject to be Indicated Names
ISHL Notified Names
Substances Subject to be Notified Names
JAN Code
P3803-100ML:4548173206424
P3803-BULK:
P3803-400ML:4548173206431
P3803-VAR:
P3803-100ML-PW:
P3803-400ML-PW:
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Philippe Lefrançois et al.
BMC genomics, 10, 37-37 (2009-01-23)
Short-read high-throughput DNA sequencing technologies provide new tools to answer biological questions. However, high cost and low throughput limit their widespread use, particularly in organisms with smaller genomes such as S. cerevisiae. Although ChIP-Seq in mammalian cell lines is replacing
Tzemach Aouizerat et al.
Bio-protocol, 10(1), e3473-e3473 (2020-01-05)
Ancient fermented food has been studied mainly based on residue analysis and recipes and reconstruction attempts were performed using modern domesticated yeast. Furthermore, microorganisms which participated in fermentation were studied using ancient-DNA techniques. In a recent paper, we presented a
Jeong-Kui Ku et al.
Annals of translational medicine, 8(6), 314-314 (2020-05-02)
The manufacturing of the demineralized dentin matrix (DDM) has been proven to extensively reduce the presence of human hepatitis B viral DNA (HBV DNA). This study measured and compared HBV DNA in fresh dentin to that in gamma radiation (GR)-sterilized
Exogenous controls increase negative call veracity in multiplexed, quantitative PCR assays for Phakopsora pachyrhizi
James Haudenshield
Plant Disease, 95.3, 343-352 (2011)
Section 1 Isolation of nucleic acids
Isabel Ferrera
Biofouling, 88-88 (2014)
Related Content
Product Information Sheet
-
Hello, I have used in the past P2069, which needs the equilibration buffer for pH8. Does P3803 also need the same equilibration buffer B5658-1EA? Thank you!
1 answer-
Helpful?
-
-
I received two bottles. One for the phenol and one for the buffer. Do I need to add the phenol to the buffer? or the buffer already contains the phenol?
1 answer-
Yes, the equilibration buffer is to be added before use, according to the following instructions:
Add the entire contents of the small bottle of Equilibration Buffer to the large bottle of Phenol:Chloroform:Isoamyl Alcohol reagent.
Mix gently and allow the phases to separate before use, typically 2–4 hours.
This will adjust the pH of the phenol phase from pH 6.7±0.2 to 8.0±0.2.Please see the link below to review the product datasheet for additional information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/490/315/p3803pis.pdfHelpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
I see that you list two equilibrated Phenol:Chloroform:Isoamyl alcohol products. When should I use Product P3803, and when should I use Product P2069?
1 answer-
In short, P3803 is for RNA work, and P2069 is for DNA work. Product P2069 consists of one bottle of Product P3803 and one bottle of Product B5658. The B5658 is added to the P3803 to raise the pH of the organic phase from 6.7 to 8.0; pH 8.0 is not suitable for RNA work, because RNA can degrade at the higher pH.
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service