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P4512

Sigma-Aldrich

Puromycin ready made solution

10 mg/mL in H2O, ≥98% (HPLC), Cell culture tested

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Synonym(s):
Achromycin, PDH, Stillomycin, Stylomycin

Quality Level

Assay

≥98% (HPLC)

form

liquid

concentration

10 mg/mL in H2O

storage temp.

2-8°C

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SBR00017B7216M5353
Puromycin ready made solution 10 mg/mL in H2O, ≥98% (HPLC), Cell culture tested

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Puromycin ready made solution

Bleomycin ready made solution Cell culture tested, 10 mg/mL in H2O, 0.2 μm filtered

B7216

Bleomycin ready made solution

Mitomycin C ready made solution 10 mg/mL in DMSO, ≥98% (HPLC), Cell culture tested

M5353

Mitomycin C ready made solution

form

liquid

form

liquid

form

liquid

form

liquid

assay

≥98% (HPLC)

assay

≥98%

assay

-

assay

≥98% (HPLC)

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

concentration

10 mg/mL in H2O

concentration

10 mg/mL in water

concentration

10 mg/mL in H2O

concentration

10 mg/mL in DMSO

General description

Puromycin is an aminonucleoside antibiotic naturally produced by Streptomyces alboniger as a tyrosyl tRNA analog. It inhibits ribosomal protein synthesis process by causing premature chain termination acting as an analog of the 3′-terminal end of the aminoacyl-tRNA. Puromycin also acts as a reversible inhibitor of dipeptidyl-peptidase II (serine peptidase) and cytosol alanyl aminopeptidase (100μM, IC50=0.6μM). Puromycin is used as a selection marker gene (puromycin-N-acetyltransferase gene, pac) in eukaryotic/mammalian cells.
Puromycin has a wide variety of biological activities with inhibitory effect against bacteria, protozoa, parasitic worms, algae, plants, and mammalian cells.
Puromycin shows broad spectrum activity, particularly strong activity against gram positive microorganisms but weak activity against acid-fast bacilli and even weaker activity against gram negative microorganisms.
Puromycin has anti-cancer properties, inducing apoptosis in breast cancer cells. It has been shown that Puromycin induces apoptosis in MCF-7 breast cancer cells using an immunological assay to study apoptosis (the modulating effect of insulin-like growth factor-1 (IGF-1)). Puromycin at 0.5 microg/ml induced a high level of apoptosis in MCF-7 breast cancer cells, although there was still a non-negligible amount of synthesized protein. The activity of Puromycin was compared to 2 other protein synthesis inhibitors (cycloheximide and emetine), in which apoptosis occurred when protein synthesis was almost completely blocked.
Puromycin also showed antitumor activity in MDA-MB-231 breast cancer cells, causing suppression of 45S pre-ribosomal RNA and upstream binding factor (UBF).
Furthermore, Puromycin was used to study the vascular smooth muscle cell viability after treatment in rabbit model.

Technical information
The suggested concentration of Puromycin in cell culture assays is 0.2-10-μg/mL. Therefore, it is recommended to dilute the ready made solution 1:1000-50,000.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2


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Ji Hoon Jung et al.
Evidence-based complementary and alternative medicine : eCAM, 2013, 879746-879746 (2013-05-22)
Since the dysregulation of ribosome biogenesis is closely associated with tumor progression, in the current study, the critical role of ribosome biogenesis related signaling was investigated in melatonin and/or puromycin induced apoptosis in MDA-MB-231 breast cancer cells. Despite its weak
S de la Luna et al.
Gene, 62(1), 121-126 (1988-01-01)
Recombinant plasmids have been obtained that lead to the accumulation of five- to ten-fold more puromycin-N-acetyl-transferase (PAC) mRNA and two- to three-fold more PAC activity than the already described plasmid pSV2pac [Vara et al., Nucl. Acids Res. 14 (1986) 4117-4124].
R Giegé et al.
Current opinion in structural biology, 8(3), 286-293 (1998-07-17)
Mimics recapitulating the structural features of tRNAs are involved in biological processes other than ribosome-dependent protein synthesis. A knowledge of the rules underlying the architecture and function of tRNAs allows the design of non-natural mimics. The study of these mimics
PUROMYCIN INHIBITION OF PROTEIN SYNTHESIS.
M A DARKEN
Pharmacological reviews, 16, 223-243 (1964-09-01)
Y Yamamoto et al.
Forensic science international, 113(1-3), 143-146 (2000-09-09)
A cytosolic alanyl aminopeptidase (AAP-S) was purified to homogeneity from human liver cytosol. The molecular weight of the purified enzyme was calculated to be approximately 98,000 on TOF-MS and 90,000 on SDS-PAGE in the presence of beta-ME. These findings suggest

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