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P8849

Sigma-Aldrich

Protease Inhibitor Cocktail

for use in purification of Histidine-tagged proteins, DMSO solution

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MDL number:
MFCD00677817
NACRES:
NA.77

biological source

synthetic

Quality Level

300

form

DMSO solution

storage temp.

−20°C

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This Item
P8340P9599P8215
Protease Inhibitor Cocktail for use in purification of Histidine-tagged proteins, DMSO solution

Sigma-Aldrich

P8849

Protease Inhibitor Cocktail

Sigma-Aldrich

Sigma-Aldrich

P8340

Protease Inhibitor Cocktail

Protease Inhibitor Cocktail for plant cell and tissue extracts, DMSO solution

Sigma-Aldrich

P9599

Protease Inhibitor Cocktail

Protease Inhibitor Cocktail for use with fungal and yeast extracts, DMSO solution

Sigma-Aldrich

P8215

Protease Inhibitor Cocktail

form

DMSO solution

form

DMSO solution

form

DMSO solution

form

DMSO solution

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

300

Quality Level

300

Quality Level

300

Quality Level

300

General description

DMSO solution designed for use in the purification of histidine-tagged proteins.

The cocktail contains a mixture of inhibitors that specifically target serine, cysteine, aspartic, and thermolysin-like proteases, and aminopeptidases.

Specificity

Inhibits serine, cysteine, aspartic, and thermolysin-like proteases, and aminopeptidases.

Application

The cocktail has been optimized and tested for isolation of histidine-tagged proteins, with chelating agents omitted for compatibility with IMAC applications.

It is recommended for inhibition of protease activity in 100 mL of cell lysate from 20 g of Escherichia coli or 10 g of baculovirus-infected Spodoptera frugiperda pupal ovary cells.

Features and Benefits

Specifically formulated for histidine-tagged protein purification.

Targets multiple types of proteases, ensuring comprehensive inhibition of protease activity.

Compatibility with IMAC applications due to omission of chelating agents.

Supplied in convenient packaging options.

Components

AEBSF
Bestatin
E-64
Pepstatin A
Phosphoramidon

Quantity

One mL is recommended for the inhibition of proteases extracted from 20 g of Escherichia coli or 10 g of baculovirus-infected Spodoptera frugiperda pupal ovary cells in a total volume of 100 ml.

This protease inhibitor cocktail has been optimized and tested for histidine-tagged proteins

Preparation Note

This product is supplied as a clear solution in DMSO. One mL of solution is recommended for inhibition of protease activity in 100 mL of cell lysate from 20 g of E. coli cells or 10 g of baculovirus-infected cells.

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P8465

Protease Inhibitor Cocktail powder, for use with bacterial cell extracts, lyophilized powder

P8340

Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution

P8215

Protease Inhibitor Cocktail, for use with fungal and yeast extracts, DMSO solution

P2714

Protease Inhibitor Cocktail powder, for general use, lyophilized powder

P9599

Protease Inhibitor Cocktail, for plant cell and tissue extracts, DMSO solution

P1860

Protease Inhibitor Cocktail, for use in tissue culture media, DMSO solution

S8820

SIGMAFAST™ Protease Inhibitor Tablets, For General Use

S8830

SIGMAFAST™ Protease Inhibitor Cocktail Tablets, EDTA-Free, for use in purification of Histidine-tagged proteins

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

185.0 °F - closed cup

Flash Point(C)

85 °C - closed cup

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

FSL

Flammable liquids
Type 3 petroleums
Hazardous rank III
Water insoluble liquid

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