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PLD35

Sigma-Aldrich

GenElute Plasmid Midiprep Kit

greener alternative

sufficient for 35 purifications

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Synonym(s):
Gen Elute, GenElute Plasmid Kit, midiprep plasmid isolation kit, plasmid extraction kit, plasmid isolation kit, plasmid purification kit
NACRES:
NA.52

usage

sufficient for 35 purifications

Quality Level

greener alternative product characteristics

Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.

technique(s)

DNA purification: suitable

greener alternative category

storage temp.

15-25°C

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This Item
PLN70PLN350PLX15
usage

sufficient for 35 purifications

usage

sufficient for 70 purifications

usage

sufficient for 350 purifications

usage

sufficient for 15 purifications

greener alternative product characteristics

Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative category

Aligned,

greener alternative category

, Aligned

greener alternative category

Aligned,

greener alternative category

Aligned,

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

The GenElute Plasmid Midiprep Kit offers a simple, rapid, cost-effective method for isolating plasmid DNA from recombinant E. coli cultures. By combining silica- binding technology and the convenience of a spin column format, up to 300 μg of plasmid DNA can be recovered from 20-40 ml of Luria Broth (LB) in about 45 minutes. Note that actual yield and optimal volume of culture to use depend on the plasmid and the culture medium.

An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto silica in the presence of high salts. Contaminants are then removed by a spin-wash step. Finally, the bound DNA is eluted in water or Tris-EDTA buffer.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Application

The DNA is ready for immediate use in such downstream applications as restriction enzyme digestion, cloning, PCR, transcription and automated sequencing.
The recovered plasmid DNA is predominately in its supercoiled form. There is no visual evidence of genomic DNA or RNA contamination detected by agarose gel electrophoresis. The DNA is ready for immediate use in downstream applications such as restriction digestion, ligation, sequencing, PCR, and transfection.

Other Notes

GenElute Plasmid Midi- and Maxiprep Kits offer a simple, rapid and cost-effective method for isolating plasmid DNA from E. coli cultures. These kits combine silica-based membrane technology with the convenience of a spin column format and deliver up to 350 μg (Midi) and 1.2 mg (Maxi) yield of high-copy plasmid DNA.

Principle

Bacterial cells are harvested via centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorbtion of DNA onto silca in the presence of high salts. Contaminants are then removed by a simple wash step. Finally, the bound DNA is eluted in water or Tris-EDTA buffer. The recovered plasmid DNA is predominately in its supercoiled form. There is no visual evidence of genomic DNA or RNA contamination.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Central nervous system

Storage Class Code

3 - Flammable liquids

WGK

WGK 2


Regulatory Listings

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PDSCL

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PRTR

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FSL

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ISHL Indicated Name

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ISHL Notified Names

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Cartagena Act

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JAN Code

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Tamás Raskó et al.
Nucleic acids research, 38(20), 7155-7166 (2010-07-01)
The GGCC-specific restriction endonuclease BspRI is one of the few Type IIP restriction endonucleases, which were suggested to be a monomer. Amino acid sequence information obtained by Edman sequencing and mass spectrometry analysis was used to clone the gene encoding
Wen Chen et al.
Journal of cellular physiology, 235(2), 1674-1688 (2019-08-23)
Renal interstitial fibrosis is a key factor in the development of chronic renal diseases, possibly leading to uremia. The present study conducted aimed to assess the hypothesis whether keratin 1 (KRT1) silencing could suppress kidney interstitial fibrosis and glomerular sclerosis
Martina Herrmann et al.
Applied and environmental microbiology, 75(10), 3127-3136 (2009-03-24)
Communities of ammonia-oxidizing archaea (AOA) and bacteria (AOB) in freshwater sediments and those in association with the root system of the macrophyte species Littorella uniflora, Juncus bulbosus, and Myriophyllum alterniflorum were compared for seven oligotrophic to mesotrophic softwater lakes and

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