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QPBCA

Sigma-Aldrich

QuantiPro BCA Assay Kit

for 0.5-30 μg/ml protein

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Synonym(s):
BCA Assay, Protein determination by BCA, bicinchoninic acid reagent
NACRES:
NA.84

Quality Level

shipped in

wet ice

storage temp.

2-8°C

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This Item
BCA1B9643DUO92010
QuantiPro™ BCA Assay Kit for 0.5-30 μg/ml protein

QPBCA

QuantiPro BCA Assay Kit

Supelco

B9643

Bicinchoninic Acid solution

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

storage temp.

−20°C

Application

In addition to protein determination and quantification in solution, the QuantiPro BCA Assay Kit has other applications, including determination of protein covalently bound to agarose supports and protein adsorbed to multiwell plates. It has also been applied to the determination of functional groups such as sulfhydryl, N-hydroxysuccinimido carboxylate, aldehyde and hydrazide on a variety of solid supports.
The kit can be used to measure very dilute protein concentrations in very small sample volumes. Accurately measures protein concentrations from 0.5 to 30 μg/mL in tube assays and 1 to 20 μg/mL in 96- or 384-well plate assays.

Features and Benefits

  • Accurate across a broad range of protein concentrations
  • High sensitivity; linear response form 0.5 to 30 μg/ml of protein
  • Stable color complex
  • Reduced susceptibility to detergents

Principle

Based on the alkaline reduction of Cu2+ to Cu1+ by proteins, and the formation of a bicinchoninic acid:Cu1+ complex having an absorbance maximum at 562 nm.

Storage and Stability

Store Reagents QA, QB, and QC at room temperature. Reagents QA and QB can be mixed together without Reagent QC (4% Copper(II) Sulfate Pentahydrate Solution 12 mL) and stored in a closed container at room temperature for a week. If this kit is received or stored cold, a precipitate may form in Reagent QA or Reagent QB. To dissolve the precipitate, warm the solution slowly to room temperature while mixing. The solutions are now suitable for use.

Legal Information

QuantiPro is a trademark of Sigma-Aldrich Co. LLC

Kit Components Only

Product No.
Description

  • Copper(II) sulfate solution, 4 % (w/v) (prepared from copper (II) sulfate pentahydrate) 12 mL

Kit Components Also Available Separately

Product No.
Description
SDS

  • P0914Protein Standard Solution: 1.0 mg/ml bovine serum albumin in 0.15 M NaCl with 0.05% sodium azide (flame-sealed glass ampules) 10 x 1SDS

Application

Product No.
Description
Pricing

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Pictograms

CorrosionEnvironment

Signal Word

Danger

Hazard Statements

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Irrit. 2

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Please refer to KIT Component information

PRTR

Please refer to KIT Component information

FSL

Please refer to KIT Component information

ISHL Indicated Name

Please refer to KIT Component information

ISHL Notified Names

Please refer to KIT Component information

Cartagena Act

Please refer to KIT Component information

JAN Code

キットコンポーネントの情報を参照してください


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Related Content

A wide variety of products for traditional protein quantitation techniques such as BCA and Bradford. Also, products for alternative assays such as Lowry, Micro Pyrogallol and FluoroProfile are also available for total protein determination.

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