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R0883

Sigma-Aldrich

RPMI-1640 Medium

With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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Synonym(s):
Roswell Park Memorial Institute 1640 medium
MDL number:
NACRES:
NA.75

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

sodium pyruvate: no
phenol red: yes
L-glutamine: no
NaHCO3: yes
HEPES: no

shipped in

ambient

storage temp.

2-8°C

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This Item
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Sigma-Aldrich

Sigma-Aldrich

R0883

RPMI-1640 Medium

Sigma-Aldrich

Sigma-Aldrich

R7638

RPMI-1640 Medium

RPMI-1640 Medium With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

R8758

RPMI-1640 Medium

SAFC

SAFC

R5886

RPMI-1640 Medium

form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

components

sodium pyruvate: no
phenol red: yes
L-glutamine: no
NaHCO3: yes
HEPES: no

components

sodium pyruvate: no
HEPES: 20 mM
L-glutamine: no
NaHCO3: yes
phenol red: yes

components

NaHCO3: yes
phenol red: yes
sodium pyruvate: no
HEPES: no
L-glutamine: yes

components

L-glutamine: no
sodium pyruvate: no
HEPES: 25 mM
phenol red: yes
NaHCO3: yes

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Application

RPMI-1640 Medium has been used:
  • to culture PC-3 prostate cell line and Jurkat cells
  • to maintain a triple-negative MDA-MB-231 and a luminal type MCF7 human Caucasian breast adenocarcinoma cell line
  • to cultivate circulating tumor cells (CTCs)

Reconstitution

Supplement with 0.3 g/L L-glutamine.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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