S2014

Staphylococcus aureus

buffered aqueous suspension, Wood 46 strain

• NACRES: NA.81

Properties

• biological source: Staphylococcus aureus
• Quality Level: 200
• sterility: Not processed or packaged aseptically
• form: buffered aqueous suspension
• composition: Cell suspension, ~10% wet weight/volume
• storage temp.: 2-8°C

Description

General description

Staphylococcus aureus Wood 46 is protein A deficient and spa negative. It shares 98% to 99% genome identity with S.aureus and shows a lower surface expression of cell wall-associated protein A.

Application

(Not intended for use as a starter culture.)

Staphylococcus aureus has been used:

• to mimic infection and induce fever in Pekin duck
• to test its effect on hemocyte morphology in hemolymph samples from beetle Tenebrio molitor larva
• in the antibacterial activity and minimum inhibitory concentration (MIC) assay with gedunin and 7-deacetoxy-7αhydroxygedunin potassium salt

Wood 46, a non-protein A producing S. aureus strain, prepared by the same method as P7155 (Protein A, crude cell suspension-Cowan strain), may be used as a control in protein A-immunoglobulin binding studies.

Packaging

10, 50 mL in serum bottle

Biochem/physiol Actions

Staphylococcus aureus Wood 46 displays reduced virulence compared to the S. aureus. This isolate is useful in understanding protein A role in pathogenesis and virulence.

Physical form

Formalin-fixed crude cell suspension of essentially non-viable S. aureus (Wood 46 strain) in 0.05 M potassium phosphate buffer, pH 7.5, containing 0.2% sodium azide

Preparation Note

Produced in pure culture.

Analysis Note

This strain binds less than 10% of the rabbit IgG bound by P 7155 as assayed by a modification of the method of Kessler.

Safety Information

• Storage Class Code: 6.2 - Infectious substances
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable