Merck
All Photos(1)

Documents

Safety Information

S2014

Sigma-Aldrich

Staphylococcus aureus

buffered aqueous suspension, Wood 46 strain

Sign Into View Organizational & Contract Pricing

NACRES:
NA.81

biological source

Staphylococcus aureus

sterility

Not processed or packaged aseptically

form

buffered aqueous suspension

composition

Cell suspension, ~10% wet weight/volume

storage temp.

2-8°C

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
P2545P6031P2165
Sigma-Aldrich

Sigma-Aldrich

S2014

Staphylococcus aureus

Millipore

Millipore

P2545

Protein A–Agarose

form

buffered aqueous suspension

form

saline suspension

form

essentially salt-free, lyophilized powder

form

lyophilized powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200, 300

sterility

Not processed or packaged aseptically

sterility

-

sterility

-

sterility

-

composition

Cell suspension, ~10% wet weight/volume

composition

-

composition

-

composition

Protein, ≥70% biuret

General description

Staphylococcus aureus Wood 46 is protein A deficient and spa negative. It shares 98% to 99% genome identity with S.aureus and shows a lower surface expression of cell wall-associated protein A.

Application

(Not intended for use as a starter culture.)
Staphylococcus aureus has been used:
  • to mimic infection and induce fever in Pekin duck
  • to test its effect on hemocyte morphology in hemolymph samples from beetle Tenebrio molitor larva
  • in the antibacterial activity and minimum inhibitory concentration (MIC) assay with gedunin and 7-deacetoxy-7αhydroxygedunin potassium salt

Wood 46, a non-protein A producing S. aureus strain, prepared by the same method as P7155 (Protein A, crude cell suspension-Cowan strain), may be used as a control in protein A-immunoglobulin binding studies.

Biochem/physiol Actions

Staphylococcus aureus Wood 46 displays reduced virulence compared to the S. aureus. This isolate is useful in understanding protein A role in pathogenesis and virulence.

Physical form

Formalin-fixed crude cell suspension of essentially non-viable S. aureus (Wood 46 strain) in 0.05 M potassium phosphate buffer, pH 7.5, containing 0.2% sodium azide

Preparation Note

Produced in pure culture.

Analysis Note

This strain binds less than 10% of the rabbit IgG bound by P 7155 as assayed by a modification of the method of Kessler.

Storage Class Code

6.2 - Infectious substances

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Poisonous substance

JAN Code

S2014-50ML:4.548173210179E12
S2014-10ML:4.548173210162E12


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 1

1 of 1

M Marais et al.
Poultry science, 90(6), 1234-1238 (2011-05-21)
Poultry, like mammals and other birds, develop fever when exposed to compounds from gram-negative bacteria. Mammals also develop fever when exposed to the constituents of viruses or gram-positive bacteria, and the fevers stimulated by these different pathogenic classes have discrete
G Kronvall et al.
Infection and immunity, 3(1), 10-15 (1971-01-01)
Protein A of Staphylococcus aureus can be detected on cell walls of intact bacteria by use of radioactively labeled myeloma globulin. Of 156 strains of S. aureus, 141 (90%) contained protein A. None of 47 S. epidermidis strains was positive
S W Kessler
Journal of immunology (Baltimore, Md. : 1950), 117(5 Pt 1), 1482-1490 (1976-11-01)
Procedures are detailed for the rapid isolation of representative cell membrane antigens with protein A-bearing staphylococci as an adsorbent for IgG antibodies complexed with the antigens. Cell surface membrane proteins were radioiodinated and solubilized in nonionic detergent. Specific antisera were
Synthetic modification of gedunin and comparative antibacterial activity of gedunin and 7-deacetoxy-7alpha-hydroxygedunin potassium salt
Okhale SE, et al.
African Journal of Pure and Applied Chemistry, 6(14), 183-189 (2012)
Manasi Balachandran et al.
Genome announcements, 5(13) (2017-04-01)
Here, we report the first complete genome sequence of the Staphylococcus aureus strain Wood 46. Wood 46 has played an important role in understanding the virulence and pathogenesis of S. aureus infections. This report will assist efforts in vaccine development against

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service