Unfortunately, the formulation of Product S5442 is considered proprietary.
S5442
SyntheChol® NS0 Supplement
500 ×, synthetic cholesterol, animal component-free, sterile-filtered, aqueous solution, suitable for cell culture
SyntheChol® NS0 Supplement
Synonym(s):
Cholesterol solution
Sign In to View Organizational & Contract Pricing.
About This Item
Empirical Formula (Hill Notation):
C27H46O
CAS Number:
Molecular Weight:
386.65
UNSPSC Code:
12352211
eCl@ss:
39023139
PubChem Substance ID:
NACRES:
NA.75
MDL number:
Skip To
SMILES string
[H][C@@]12CC=C3C[C@@H](O)CC[C@]3(C)[C@@]1([H])CC[C@]4(C)[C@H](CC[C@@]24[H])[C@H](C)CCCC(C)C
InChI key
HVYWMOMLDIMFJA-DPAQBDIFSA-N
InChI
1S/C27H46O/c1-18(2)7-6-8-19(3)23-11-12-24-22-10-9-20-17-21(28)13-15-26(20,4)25(22)14-16-27(23,24)5/h9,18-19,21-25,28H,6-8,10-17H2,1-5H3/t19-,21+,22+,23-,24+,25+,26+,27-/m1/s1
biological source
non-animal source
sterility
sterile-filtered
product line
BioXtra
form
aqueous solution
concentration
500 ×
technique(s)
cell culture | mammalian: suitable
storage temp.
2-8°C
Quality Level
Gene Information
human ... POLA1(5422), TOP2A(7153)
rat ... Ar(24208), Polb(29240)
1 of 4
This Item | 1.04672 | 228111 | 47127-U |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) - | technique(s) HPLC: suitable, gas chromatography (GC): suitable |
| biological source non-animal source | biological source synthetic | biological source - | biological source - |
| form aqueous solution | form powder | form powder | form liquid |
| storage temp. 2-8°C | storage temp. 15-25°C | storage temp. 10-30°C | storage temp. -10 to -25°C |
| concentration 500 × | concentration - | concentration - | concentration 10 mg/mL in chloroform |
| Quality Level 500 | Quality Level 500 | Quality Level 100 | Quality Level 300 |
Application
Features and Benefits
Synthetic, non-animal cholesterol solution that is designed as a supplement to our hybridoma media formulations to promote excellent cell growth and protein productivity for NS0 mouse myeloma and other cholesterol-dependent cell lines.
Legal Information
SyntheChol is a registered trademark of Merck KGaA, Darmstadt, Germany
related product
Product No.
Description
Pricing
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
Substances Subject to be Indicated Names
ishl_indicated
Substances Subject to be Notified Names
ishl_notified
S5442-2ML: + S5442-VAR: + S5442PROC: + S5442-100ML: + S5442-PH: + S5442-10ML: + S5442-BULK:
jan
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Arthur Nathan Brodsky et al.
Methods (San Diego, Calif.), 56(3), 408-417 (2011-10-29)
DNA microarray analysis of gene expression has become a valuable tool for bioprocessing research aimed at improving therapeutic protein yields. The highly parallel nature of DNA microarray technology allows researchers to assess hundreds of gene simultaneously, essentially enabling genome-wide snapshots.
Suba Dharshanan et al.
Cytotechnology, 66(4), 625-633 (2013-07-25)
From our recent publications, it was found that the deimmunization method (Dharshanan et al. (2012) Sci Res Essays 7:2288-2299) should be applied for the development of humanized anti-C2 monoclonal antibody (H1C2 mAb). However, the overlapping-PCR mutagenesis procedure used to insert
Steve Schoonooghe et al.
BMC biotechnology, 9, 70-70 (2009-08-13)
Tumour associated antigens on the surface of tumour cells, such as MUC1, are being used as specific antibody targets for immunotherapy of human malignancies. In order to address the poor penetration of full sized monoclonal antibodies in tumours, intermediate sized
Marco Guadagnoli et al.
Blood, 117(25), 6856-6865 (2011-05-06)
APRIL (A proliferation-inducing ligand) is a TNF family member that binds two TNF receptor family members, TACI and BCMA. It shares these receptors with the closely related TNF family member, B-cell activating factor (BAFF). Contrary to BAFF, APRIL binds heparan
Nancy Lynn Booth et al.
Cancer immunology, immunotherapy : CII, 58(8), 1229-1244 (2008-12-18)
We have developed a high-throughput screen (HTS) to search for novel molecules that can synergize with TRAIL, thus promoting apoptosis of ACHN renal tumor cells in a combinatorial fashion. The HTS detects synthetic compounds and pure natural products that can
Articles
Discover answers to your frequently asked questions about animal component-free, xenobiotic-free, and chemically defined media and reagents for cell culture.
Related Content
-
What is the concentration of product S5442?
1 answer-
Helpful?
-
-
Is this formulation stable at room temperature? If so, how long is it stable?
1 answer-
This product ships at ambient temperature and is stable for relatively short periods (up to two weeks for transit time) at room temperature. However, refrigerated temperatures are recommended for long-term storage.
Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/397/094/s5442dat.pdfHelpful?
-
-
Could you please clarify the concentration of cholesterol in this product both at the original 500X solution and at the final 1X?
1 answer-
The concentration of synthetic cholesterol in this product is 0.25% or 2.5 mg/mL as supplied. The product is considered a 500X preparation meaning that 2 mL of this solution will supplement 1 L of culture medium. The 1X concentration, when diluted in 1L of medium, would be 5 ug/mL. Some cells may require a higher concentrations of cholesterol. The suggested usage range for this product is 2 - 6 mL per liter of medium. See the link below to review the product datasheet.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/397/094/s5442dat.pdfHelpful?
-
-
Hi, What is the concentration of Synthechol in this product in mg/ml or mol/l Thank you
1 answer-
Unfortunately, the formulation of item S5442 is proprietary. The concentration of the synthetic cholesterol is 0.25% (w/v) as supplied.
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service