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SBR00017

Sigma-Aldrich

Puromycin aminonucleoside Ready Made Solution

10 mg/mL in water

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Synonym(s):
3′-Amino-3′-deoxy-N6,N6-dimethyladenosine
Empirical Formula (Hill Notation):
C12H18N6O3
CAS Number:
Molecular Weight:
294.31
NACRES:
NA.85

Assay

≥98%

Quality Level

form

liquid

concentration

10 mg/mL in water

antibiotic activity spectrum

Gram-positive bacteria
neoplastics
parasites

Mode of action

protein synthesis | interferes

storage temp.

2-8°C

SMILES string

N[C@H]1[C@@H](O)[C@H](N(C=N2)C3=C2C(N(C)C)=NC=N3)O[C@@H]1CO

InChI

1S/C12H18N6O3/c1-17(2)10-8-11(15-4-14-10)18(5-16-8)12-9(20)7(13)6(3-19)21-12/h4-7,9,12,19-20H,3,13H2,1-2H3

InChI key

RYSMHWILUNYBFW-UHFFFAOYSA-N

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This Item
P7130P7255P9620
Puromycin aminonucleoside

P7130

Puromycin aminonucleoside

Puromycin dihydrochloride Ready Made Solution, from Streptomyces alboniger, 10 mg/mL in H2O, suitable for cell culture

P9620

Puromycin dihydrochloride

mode of action

protein synthesis | interferes

mode of action

protein synthesis | interferes

mode of action

protein synthesis | interferes

mode of action

protein synthesis | interferes

Quality Level

200

Quality Level

300

Quality Level

300

Quality Level

300

form

liquid

form

powder

form

powder

form

solution

assay

≥98%

assay

-

assay

≥98% (HPLC)

assay

>98% (HPLC)

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

Biochem/physiol Actions

Puromycin aminonucleoside is an aminonucleoside derivative of the known antibiotic puromycin. Puromycin aminonucleoside has been used in nephrology research, studying focal and segmental glomerulosclerosis, and in the induction of nephrosis in rats. Rats with puromycin aminonucleoside-induced nephrotic syndrome were used to study the excretion of sodium and NOx metabolites.

Puromycin aminonucleoside has also been used to probe endothelial glycosaminoglycan synthesis in cultured glomerular endothelial cells and their relation to cell permeability. A puromycin aminonucleoside nephrosis model of rat glomerular disease was also used to study the role of the neuron-specific ubiquitin C-terminal hydrolase protein gene product 9.5 (PGP 9.5).

Preparation Note

Puromycin aminonucleoside solution is provided at 10 mg/mL in water and could be further diluted in aqueous buffers to a working concentration of 10 μg/mL (1:1000).

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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I Shirato et al.
Journal of the American Society of Nephrology : JASN, 11(12), 2381-2386 (2000-11-30)
Parietal epithelial cells (PEC) of Bowman's capsules cover the inner aspect of Bowman's capsules and are believed to contribute to extracapillary lesions of glomerulonephritis such as crescent formation. In glomerular research including cell culture experiments and pathology, differentiation between PEC
Agnes B Fogo
Seminars in nephrology, 23(2), 161-171 (2003-04-22)
Glomerulosclerosis in a heterogeneous pattern, ie, focal and segmental glomerulosclerosis (FSGS), is a common endpoint in a variety of settings, including idiopathic FSGS, and scarring secondary to other renal or systemic diseases. These different causes contribute to the diverse clinical
Jenny Sörensson et al.
American journal of physiology. Renal physiology, 284(2), F373-F380 (2002-10-22)
It has been suggested that proteinuria is caused by alterations of the charge selectivity of the basement membrane and/or the epithelial cell layer (podocytes). However, recent findings suggest that the endothelial luminal surface coat, consisting of proteoglycans with their connected
Alicia A McDonough et al.
Current opinion in nephrology and hypertension, 12(5), 533-541 (2003-08-16)
The proximal tubule sodium/hydrogen exchanger continuously reabsorbs the bulk of the filtered sodium, controlling salt delivery to the distal nephron which is critical for tubuloglomerular feedback autoregulation and for fine control of salt excretion in the distal nephron. This review
Marta Bermejo-Jambrina et al.
Frontiers in immunology, 11, 2010-2010 (2020-09-15)
Dendritic cells (DCs) possess intrinsic cellular defense mechanisms to specifically inhibit HIV-1 replication. In turn, HIV-1 has evolved strategies to evade innate immune sensing by DCs resulting in suboptimal maturation and poor antiviral immune responses. We previously showed that complement-opsonized

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