SBR00022

Blasticidin S Ready Made Solution

10 mg/mL (20 mM HEPES), 0.22 μm filtered

• Empirical Formula (Hill Notation): C₁₇H₂₇N₈O₅Cl
• Molecular Weight: 458.90

Properties

• sterility: 0.22 μm filtered
• concentration: 10 mg/mL (20 mM HEPES)
• antibiotic activity spectrum: fungi
• Mode of action: protein synthesis | interferes
• storage temp.: −20°C

Description

Biochem/physiol Actions

Blasticidin S belongs to the aminoacylnucleoside class of antibiotics. It inhibits protein synthesis in bacteria and eukaryotes. It also has fungicidal properties and prevents rice blast disease. Blasticidin S inhibits hydrolysis of peptidyl-tRNA induced by release factors. It enhances the binding of tRNA to the large subunit of ribosomes and to an extent inhibits peptide bond formation. Blasticidin S is also used as a selection agent for transformed cells that contain the resistance genes bls, bsr, or bsd. Blasticidin S has been used to select HEK293-T cells with TLR-2 constructs and HEK-D5 cells. It is also used to study protein synthesis at the level of peptide bond formation.
Working stock: The suggested concentration of Blasticidin S in cell culture assays is 10μg/mL. Therefore, it is recommended to dilute the readymade solution 1:1000.

Safety Information

• Pictograms: GHS06
• Signal Word: Danger
• Hazard Statements: H301
• Precautionary Statements: P264 - P270 - P301 + P310 - P405 - P501
• Hazard Classifications: Acute Tox. 3 Oral
• Storage Class Code: 6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable