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SBR00022

Sigma-Aldrich

Blasticidin S Ready Made Solution

10 mg/mL (20 mM HEPES), 0.22 μm filtered

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Empirical Formula (Hill Notation):
C17H27N8O5Cl
Molecular Weight:
458.90
UNSPSC Code:
12352106
NACRES:
NA.76

Quality Level

sterility

0.22 μm filtered

Assay

≥98% (HPLC)

form

liquid

concentration

10 mg/mL (20 mM HEPES)

color

colorless to faint yellow

antibiotic activity spectrum

fungi

Mode of action

protein synthesis | interferes

storage temp.

−20°C

SMILES string

CN(CCC(CC(=O)NC1C=CC(OC1C(=O)O)N2C=CC(=NC2=O)N)N)C(=N)N.Cl

InChI

1S/C17H26N8O5.ClH/c1-24(16(20)21)6-4-9(18)8-12(26)22-10-2-3-13(30-14(10)15(27)28)25-7-5-11(19)23-17(25)29;/h2-3,5,7,9-10,13-14H,4,6,8,18H2,1H3,(H3,20,21)(H,22,26)(H,27,28)(H2,19,23,29);1H

InChI key

YQXYQOXRCNEATG-UHFFFAOYSA-N

Related Categories

General description

Blasticidin S, originally isolated from S. griseochromogenes, is a bacterial metabolite renowned for its antibacterial and fungicidal activities. This compound serves as a potent inhibitor of protein synthesis, exhibiting activity against various microorganisms, including bacteria (B. subtilis, S. lutea, E. coli, P. fluorescens, and M. tuberculosis), tumor cell lines, and nematodes.

In research, Blasticidin S has become a valuable tool, notably as a marker for strain manipulations. Recent applications involve the use of Blasticidin S as a selection agent for cells carrying plasmids conferring blasticidin resistance. The resistance is mediated by the blasticidin S deaminase genes (bsr from Bacillus cereus or BSD from Aspergillus terreus). These genes produce enzymes that catalyze the hydrolytic deamination of the cytosine moiety in blasticidin S, leading to the formation of a non-toxic deaminohydroxy derivative. This resistance mechanism is crucial in various studies involving genetic manipulations and selections in cell biology and biochemical research.

Application

Blasticidin S has been:
  • studies to have fungicidal properties and prevents rice blast disease.
  • used as a selection agent for transformed cells that contain the resistance genes bls, bsr, or bsd. Blasticidin S has been used to select HEK293-T cells with TLR-2 constructs and HEK-D5 cells.
  • used to study protein synthesis at the level of peptide bond formation.

Biochem/physiol Actions

Mode of Action: It inhibits protein synthesis in bacteria and eukaryotes. Blasticidin S inhibits hydrolysis of peptidyl-tRNA induced by release factors. It enhances the binding of tRNA to the large subunit of ribosomes and to an extent inhibits peptide bond formation.

Antimicrobial Spectrum: Active against mycobacteria, several Gram-positive and Gram-negative bacteria

Features and Benefits

  • High-quality antibiotic suitable for multiple research applications
  • Ideal for Cell Biology and Biochemical research

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Pictograms

Skull and crossbones

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 3 Oral

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Deleterious substance

JAN Code

SBR00022-1ML-PW:
SBR00022-VAR:
SBR00022-10ML:4548173363776
SBR00022-1ML:4548173363783
SBR00022-BULK:
SBR00022-10ML-PW:


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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