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T9424

Sigma-Aldrich

TRI Reagent®

For processing tissues, cells cultured in monolayer or cell pellets

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Synonym(s):
DNA/RNA/protein extraction reagent, DNA/RNA/protein isolation reagent, DNA/RNA/protein purification reagent, TRI Reagent® cell pellet solution, cell pellet RNA extraction, cell pellet RNA isolation, cell pellet RNA purification, single step RNA extraction reagent, single step RNA isolation reagent, single step RNA purification reagent, total RNA extraction solution, total RNA isolation solution, total RNA purification solution, TRI Reagent® RNA Isolation Reagent
MDL number:
NACRES:
NA.52

Quality Level

usage

 mL sufficient for 107 cells
 mL sufficient for 100 mg tissue (or)

storage temp.

2-8°C

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This Item
T3934TRIPURE-RO93289
vibrant-m

T9424

TRI Reagent®

vibrant-m

T3934

TRI Reagent®

vibrant-m

TRIPURE-RO

TriPure Isolation Reagent

vibrant-m

93289

TRI Reagent®

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

storage temp.

-

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

100

General description

TRI reagent® is a quick and convenient ready-to-use reagent useful for efficient total RNA extraction or for the simultaneous isolation of RNA, DNA and protein. TRI reagent® is a mixture of guanidine thiocyanate and phenol in a monophasic solution.
Homogenization or lysis of the tissue sample in TRI reagent® dissolves RNA, DNA and protein. The addition of chloroform or 1-bromo-3-chloropropane followed by centrifugation results in the separation of the mixture into three phases: an aqueous phase containing the RNA, the interphase containing DNA, and an organic phase containing proteins. Each of the components can then be isolated after separating the phases.

Application

TRI Reagent has been used:
  • in the isolation of total RNA from various tissue samples and cells
  • for the lysis of monocytes
  • to isolate cytoplasmic RNA from SARS-CoV infected Vero cells
  • to isolate total RNA from MDMs (monocyte-derived macrophages) infected with influenza A (H5N1) virus
  • to prepare a viral lysate of SARS-CoV culture for the creation of a clone encoding the S glycoprotein in the development of SARS-CoV vaccine

The RNA, DNA and protein that are isolated using TRI reagent® can further be used for downstream applications, such as cloning, PCR, RT-PCR, Northern blots, mRNA isolation, in vitro translation, RNase protection assay, restriction enzyme digestion, Southern blots, SDS-PAGE and western blots.
TRI Reagent is an improved version of the single-step total RNA isolation reagent developed by Chomczynski. The RNA isolation method based on this reagent is widely used and proven for RNA applications. It is ideal for quick, economical, and efficient isolation of total RNA or the simultaneous isolation of RNA, DNA, and proteins from samples of human, animal, plant, yeast, bacterial, and viral origin.

Features and Benefits

Easy-to-use: A single reagent that can be used for RNA, DNA and protein isolation that is free of any contamination.
Multi-purpose: TRI Reagent® performs well with large or small amounts of tissue or cells and works with many samples including human, plant, yeast, bacterial and viral samples.
Efficient: TRI reagent® gives better yields than traditional guanidine thiocyanate/cesium chloride methods. The whole process of RNA extraction starting with fresh tissue or cells can be completed in less than one hour.
  • Easily scalable RNA isolation
  • Works with many sources: human, plant, yeast, bacterial, or viral
  • Better yields than traditional guanidine thiocyanate/cesium chloride methods

Legal Information

TRI Reagent is a registered trademark of Molecular Research Center, Inc.

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

Supplementary Hazards

Storage Class Code

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 2

Flash Point(F)

174.2 °F - closed cup

Flash Point(C)

79 °C - closed cup


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Deleterious substance

FSL

Flammable liquids
Type 3 petroleums
Hazardous rank III
Water insoluble liquid

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

T9424-200ML:4.54817321458E12
T9424-25ML:4.548173214597E12
T9424-100ML:4.548173214573E12


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Articles

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

Protocols

TRI Reagent is a single-step RNA isolation reagent that allows simultaneous isolation of DNA, RNA and protein. This protocol outlines sample prep as well as extraction of the various samples and troubleshooting.

Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

Related Content

An overview of cell lysis and protein extraction methods including detergent solubilization, freeze-thaw lysis, osmotic shock, sonication, enzymatic cell lysis, and mechanical disruption techniques such as Dounce, Polytron, and mortar and pestle homogenization.

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