This product is deionized and distilled during production, but it is not guaranteed to be completely ion-free as ICP analysis is not performed. This product is sterilized by 0.2 µm sterile filtration rather than autoclaving. Sterility testing is performed and confirmed in the product Certificate of Analysis. Please see the link below to review a sample or lot-specific Certificate:
https://www.sigmaaldrich.com/product/sigma/w3500
W3500
Water
sterile-filtered, BioReagent, suitable for cell culture
Synonym(s):
Cell culture grade water, Ultra-filtered Water
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| Size/SKU | Availability | Price |
|---|---|---|
100 mL | Estimated to ship TODAYfrom市川市大町368 | ¥2,210 |
500 mL | Estimated to ship TODAYfrom市川市大町368 | ¥2,520 |
1 L | Estimated to ship TODAYfrom市川市大町368 | ¥4,360 |
6 x 500 mL | Estimated to ship onNovember 18, 2026from市川市大町368 | ¥10,400 |
6 x 1 L | Estimated to ship onNovember 18, 2026from市川市大町368 | ¥27,300 |
24 x 500 mL | Estimated to ship onNovember 18, 2026from市川市大町368 | ¥59,500 |
About This Item
Linear Formula:
H2O
CAS Number:
Molecular Weight:
18.02
UNSPSC Code:
12352207
NACRES:
NA.75
PubChem Substance ID:
EC Number:
231-791-2
Beilstein/REAXYS Number:
2050024
MDL number:
Technique(s):
cell culture | mammalian: suitable
Bp:
100 °C (lit.)
Vapor pressure:
3 mmHg
vapor density
<1 (vs air)
Quality Segment
vapor pressure
3 mmHg
sterility
sterile-filtered
product line
BioReagent
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/mL endotoxin
refractive index
n20/D 1.34 (lit.)
pH
5-7
bp
100 °C (lit.)
mp
0 °C (lit.)
density
1.000 g/mL at 3.98 °C (lit.)
SMILES string
O
InChI
1S/H2O/h1H2
InChI key
XLYOFNOQVPJJNP-UHFFFAOYSA-N
General description
This product is a very low endotoxin (less than 1 EU/mL), 0.2 micron filtered.
Application
For use in the preparation of cell culture media, and cell suspension and washing solutions.
Other Notes
Easily compare specifications for Water products with the Water specification table
1 of 1
This Item | |||
|---|---|---|---|
| description sterile-filtered, BioReagent, suitable for cell culture | description Molecular Biology, sterile filtered | description for embryo transfer, sterile-filtered, BioXtra, suitable for mouse embryo cell culture | description suitable for ion chromatography |
| technique(s) cell culture | mammalian: suitable | technique(s) FISH: suitable | technique(s) cell culture | embryo: suitable | technique(s) ion chromatography: suitable |
| Quality Level 400 | Quality Level 100 | Quality Level 200 | Quality Level 100 |
| form liquid | form liquid | form liquid | form liquid |
| bp 100 °C (lit.) | bp 100 °C (lit.) | bp 100 °C (lit.) | bp 100 °C (lit.) |
| refractive index n20/D 1.34 (lit.) | refractive index n20/D 1.34 (lit.) | refractive index n20/D 1.34 (lit.) | refractive index n20/D 1.34 (lit.) |
| impurities ≤1 EU/mL endotoxin | impurities phosphatases, none detected, DNases, none detected, proteases, none detected, RNases, none detected | impurities endotoxin, tested | impurities ≤10 μg/kg acetate, ≤10 μg/kg oxalate, ≤10 μg/kg formiate, ≤10 μg/kg glycolate |
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Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
W3500-1L-CO: + W3500-2L: + W3500-6X1L: + W3500-500ML-C: + W3500-500ML: + W3500-100ML-C: + W3500-VAR: + W3500PROC: + W3500-1L: + W3500-100ML: + W3500-24X500ML: + W3500-PH: + W3500-6X500ML: + W3500-BULK: + CR1104-PROC: + W3500-1LSTAB:
jan
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Is it deionized? How is it sterilized?
1 answer-
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Is this product deionized?
1 answer-
This product is deionized and distilled during production. However, it is not subjected to ICP analysis, so it is not warranted to be ion-free.
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How is this product used?
1 answer-
This product is tested for use in cell culture. Parameters such as cytotoxicity, pH, and endotoxin are measured. It may be used for any general application but is of most interest to customers doing cell culture work. It has not been tested for RNAse, DNAse, or protease activity.
Please see the link below to review a sample Certificate of Analysis:
https://www.sigmaaldrich.com/coa/SIGMA/W3500/RNBL9398Helpful?
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