The conductivity of this water is not determined. Currently, there is no Molecular Biology grade water product available that is tested for conductivity.
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About This Item
electrophoresis: suitable
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Product Name
Water, Nuclease-Free Water, for Molecular Biology
vapor density
<1 (vs air)
Quality Level
vapor pressure
3 mmHg
sterility
0.1 μm filtered
form
liquid
technique(s)
PCR: suitable
electrophoresis: suitable
refractive index
n20/D 1.34 (lit.)
pH
5-7
bp
100 °C (lit.)
mp
0 °C (lit.)
density
1.000 g/mL at 3.98 °C (lit.)
foreign activity
DNase, RNase, Protease, free
SMILES string
O
InChI
1S/H2O/h1H2
InChI key
XLYOFNOQVPJJNP-UHFFFAOYSA-N
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This Item | 9801-OP | 95289 | 95284 |
|---|---|---|---|
| technique(s) PCR: suitable, electrophoresis: suitable | technique(s) - | technique(s) - | technique(s) FISH: suitable |
| Quality Level 200 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| form liquid | form liquid | form liquid | form liquid |
| mp 0 °C (lit.) | mp 0 °C (lit.) | mp 0 °C (lit.) | mp 0 °C (lit.) |
| refractive index n20/D 1.34 (lit.) | refractive index n20/D 1.34 (lit.) | refractive index n20/D 1.34 (lit.) | refractive index n20/D 1.34 (lit.) |
| density 1.000 g/mL at 3.98 °C (lit.) | density 1.000 g/mL at 3.98 °C (lit.) | density 1.000 g/mL at 3.98 °C (lit.) | density 1.000 g/mL at 3.98 °C (lit.) |
Application
Features and Benefits
- Nuclease-Free Water
- DNase-Free, RNase-Free, Protease- Free
- Not DEPC Treated
- 0.1 um Filtered
- Prepared from Double Distilled and Deionized Water
- Suitable for use in Molecular Applications
This product is 0.1um filtered. It is designated as a Molecular Biology Reagent and is suitable for molecular biology applications. This product has been analyzed for the absence of nucleases and proteases, and has undergone bioburden analysis. It has not been treated with diethyl pyrocarbonate (DEPC).
Preparation Note
Other Notes
related product
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
JAN Code
W4502-100ML:
W4502-50ML-KC:
W4502-EW:
W4502-1L:
W4502-VAR:
W4502-1L-C:
W4502-50ML:
W4502-6X1L:
W4502-6X1L-I-PW:
W4502-BULK:
W4502PROC:
W4502-20ML-KC:
W4502-10L:4548173215167
W4502-25ML:
W4502-10ML:
W4502-6X1L-I:
W4502-10ML-KC:
W4502-1ML-KC:
W4502-5ML:
W4502-10X50ML:
W4502-20ML:
W4502-1ML:
W4502-3ML:
W4502-100ML-KC:
W4502-5ML-KC:
W4502-25ML-KC:
W4502-3ML-KC:
W4502-1.5ML:
W4502-1.5ML-KC:
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Articles
Understand how mRNA vaccines induce immunity. and read how synthetic mRNA is prepared for vaccine immunogens and other biopharmaceuticals. Find reagents for synthesis of mRNA.
The assessment of DNA quality is a crucial first step in acquiring meaningful data from formalin-fixed paraffin-embedded (FFPE) tissues, and other sources of damaged DNA. Using intact genomic DNA is key for successful analysis of chromosomal aberrations (e.g. SNP analysis, LOH, aCGH, etc.).
mRNAワクチンがどのように免疫を誘導するのかを理解し、ワクチン免疫原やバイオ医薬品用に合成mRNAがどのように調製されるのかご覧ください。mRNA合成用試薬についてご紹介します。
Protocols
mRNA遺伝子発現を分析する逆転写には、転写物の半減期のばらつきや時間的パターンなどの課題が伴います。
qPCRにおけるSYBR Green I色素は、ターゲット量を測定し、これは、標準プロトコルで特定のニーズに適合させることができます。
遺伝子発現データの解析には、安定的なリファレンスまたはローディングコントロールが必要です。通常は、1つ以上のリファレンス遺伝子をリファレンスとして使用します
Standard qPCR protocol with up to four detection probes at specified concentrations, simplifying reaction setup with LuminoCt ReadyMix.
Related Content
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W4502 Millipore Water: what is the ohms of this water?
1 answer-
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Do you have stability data for W4502 for evaluating if Shelf Life can be extended?
1 answer-
This water product is not assigned an expiration or retest date. In the original sealed container the material is stable nearly indefinitely. Items that are not assigned an expiration date are warranteed for 1 year from the date of shipment.
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Is this water sterile? Can this be used for cell culture?
1 answer-
Product W4502 is not sterile, this product is prepared from Double Distilled and Deionized Water. This product is best suitable to use in molecular biology applications. See product W3500 for Sterile, Tissue Culture grade Water:
https://www.sigmaaldrich.com/product/sigma/w3500Helpful?
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In what vessel does this product come? A bottle, a bag with a spigot, etc.?
1 answer-
This product is packaged in square PET square bottles. To request a quote for larger volumes or specialized packaging, please submit a request at the link below:
https://www.sigmaaldrich.com/services/support/bulk-quotation-requestHelpful?
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This product does not list an expiration date on the label. Could someone provide an expiration for lot number BCCH7145?
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This product does not contain an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services
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