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Z374873

PCR microtubes with attached caps

capacity 0.2 mL

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UNSPSC Code:
41121702
NACRES:
NB.22

material

colorless cap
colorless tube
conical bottom
flat cap
polypropylene
polypropylene cap
polypropylene tube

description

Autoclavable

sterility

non-sterile

feature

RNase and DNase free
autoclavable at 121 °C
graduations: no
Push fit

packaging

pack of 250 ea
case of 4 packs

technique(s)

PCR: suitable

capacity

0.2 mL

color

clear

suitability

suitable for (PCR applications)

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General description

Thin-walled for efficient thermal transfer and shorter cycle times; fits all leading thermal cyclers including PerkinElmer, Biometra, MJ Research, Techne, Grant and Strategene (0.65 mL only). Certified DNase- and RNase-free. All tolerate organic solvent reactions and temperatures from -4 to 121 °C.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.

Hot Start dNTPs block DNA polymerase until heat activation, enhancing PCR specificity.

Perform reverse transcription (RT) using a reverse transcriptase enzyme and dNTPs. Use total RNA or a gene-specific approach so that only the RNA of interest is converted to cDNA.

Standard qPCR protocol with up to four detection probes at specified concentrations, simplifying reaction setup with LuminoCt ReadyMix.

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