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Z374903

Sigma-Aldrich

PCR multiwell plates

96 well plate for PCR

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Synonym(s):
96 mulitwell PCR plate, 96 well PCR microplate, 96 well PCR plate, 96 well microtiter plate
UNSPSC Code:
41106300
NACRES:
NB.22

material

colorless polypropylene
colorless wells

sterility

non-sterile

feature

plate format: 96 well standard

packaging

case of 25 ea

manufacturer/tradename

Sorenson 2633

technique(s)

PCR: suitable

size

96 well

well maximum volume

300 μm

working volume

200 μL

suitability

suitable for (PCR, RT-PCR or DNA purification applications)

application(s)

agriculture

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General description

A rigid top plate (included) minimizes plate distortion, assures a dependable fit with the PCR thermal cycler, and allows for a leak-proof seal with micro-mats or cap strips. Each well has a maximum capacity of 200 μL.

Features and Benefits

  • Virgin polypropylene
  • Fully autoclavable
  • Certified DNase- and RNase-free
  • Wells have thin walls for rapid temperature equilibration and reduced cycle time

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.

Hot Start dNTPs block DNA polymerase until heat activation, enhancing PCR specificity.

Perform reverse transcription (RT) using a reverse transcriptase enzyme and dNTPs. Use total RNA or a gene-specific approach so that only the RNA of interest is converted to cDNA.

Standard qPCR protocol with up to four detection probes at specified concentrations, simplifying reaction setup with LuminoCt ReadyMix.

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