Z734438
ThermalSeal RTS™ Sealing Films
for qPCR, storage & crystallization, non-sterile
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material
polyolefin film
silicone/adhesive
sterility
non-sterile
packaging
pack of 100 ea
manufacturer/tradename
Excel Scientific TSS-RTQ-100
technique(s)
PCR: suitable
qPCR: suitable
L × W
76.2 mm × 133.4 mm
W × L
7.62 cm × 13.34 cm
color
clear
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General description
ThermalSeal RTS™ films are sized to fit within the edges of raised-rim 96-well plates. Their consistent high optical clarity makes possible reproducible, reliable, and consistent DNA amplification measurements and crystal detection. Two end tabs assist in positioning the film on the plate, and the non-tacky adhesive surface simplifies handling. Easy removal of the end tabs at perforated boundaries prevents lifting and higher evaporation rates that can occur with films that overlap the plate rim.
Dimensions 76.2 by 133.4 mm. With end tabs removed, length is 113.0 mm.
- High optical clarity
- Minimal to no autofluorescence
- Chemically inert; no extractables except at extreme pH
- DMSO resistant for HTS
- Heat resistant, recommended for temperatures from
- -70°C to +100°C
- Certified DNase, RNase, and Nucleic Acid free
- Fit within raised plate rim to prevent loss of seal due to film lifting
- Silicone adhesive forms the strongest available seal for evaporation prevention
- Non-tacky adhesive layer simplifies handling of film prior to sealing
Legal Information
Certificates of Analysis (COA)
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Protocols
Perform reverse transcription (RT) using a reverse transcriptase enzyme and dNTPs. Use total RNA or a gene-specific approach so that only the RNA of interest is converted to cDNA.
Standard qPCR protocol with up to four detection probes at specified concentrations, simplifying reaction setup with LuminoCt ReadyMix.
Reverse transcription analyzes mRNA gene expression, facing challenges like transcript half-life differences and temporal patterns.
qPCR protocol template with specific detection probes aids in single-target detection using LuminoCt® ReadyMix™.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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