Merck
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ZRB1019

Sigma-Aldrich

Anti-Fibrillin-1 Antibody, clone 6C19 ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

Synonym(s):
FBN1

biological source

rabbit

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

clone

recombinant monoclonal

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 312.3 kDa
observed mol wt ~35 kDa

species reactivity

human

species reactivity (predicted by homology)

monkey

packaging

antibody small pack of 25 μL

greener alternative product characteristics

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Designing Safer Chemicals
Design for Energy Efficiency
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enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

affinity binding assay: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 6C19 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Fibrillin-1. It targets an epitope within the N-terminal half.

Immunogen

GST/His-tagged recombinant fragment corresponding to 95 amino acids from the N-terminal half of human Fibrillin-1.

Application

Quality Control Testing
Western Blotting Analysis: A 1:1000 dilution of this antibody detected GST/His-tagged recombinant human Fibrillin-1.Tested applicationsImmunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Fibrillin-1 in human placenta tissue sections.Affinity Binding Assay: A representative lot of this antibody bound Fibrillin 1 recombinant fragment with a KD of 1.7 x 10-6 in an affinity binding assay.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Fibrillin-1, clone 6C19 ZooMAb, Cat. No. ZRB1019, is a recombinant Rabbit monoclonal antibody that specifically targets Fibrillin-1 and has been tested for use in Affinity Binding Assay, Immunohistochemistry (Paraffin), and Western Blotting.

Target description

Fibrillin-1 (UniProt: P35555; also known as FBN1) is encoded by the FBN1 (also known as FBN) gene (Gene ID: 2200) in human. Fibrillin-1 is a structural component of the 10-12 nm diameter microfibrils of the extracellular matrix that conveys both structural and regulatory properties to load-bearing connective tissues. Fibrillin-1-containing microfibrils provide long-term force bearing structural support. Fibrillin-1 is synthesized with a signal peptide (aa 1-24) and a propeptide (aa 25-44) that are cleaved off to generate the mature Fibrillin-1, which can be further cleaved by furin between residues 2731 and 2732 to produce asprosin (aa 2732-2871). Asprosin serves as a hormone that targets the liver to increase plasma glucose levels. It promotes glucose release from hepatocytes by activating protein kinase A. Fibrillin-1 is reported to play a role in tissue homeostasis through specific interactions with growth factors, such as the bone morphogenetic proteins (BMP), growth and differentiation factors, and latent TGF-beta binding proteins. It is also reported to regulate osteoblast maturation by controlling TGF-beta bioavailability and calibrating TGF-beta and BMP levels. It is also shown to negatively regulate osteoclastogenesis by binding and sequestering an osteoclast differentiation and activation factor TNFSF11. Mutations in FBN1 gene are known to cause Marfan syndrome, a disorder of the connective tissue that affects skeletal, ocular, and cardiovascular systems. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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