Merck
All Photos(8)

ZRB1066

Sigma-Aldrich

Anti-PNPT1 Antibody, clone 2D23 ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

Synonym(s):
3′-5′ RNA exonuclease OLD35, EC:2.7.7.8, PNPase 1, PNPase old-35, Polynucleotide phosphorylase 1, Polynucleotide phosphorylase-like protein, Polyribonucleotide nucleotidyltransferase 1 mitochondrial

biological source

rabbit

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

clone

recombinant monoclonal

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 85.95 kDa
observed mol wt ~80 kDa

species reactivity

mouse, human, rat

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

affinity binding assay: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 2D23 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects PNPase 1 (PNPT1). It targets an epitope within 20 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal region of human PNPase 1 (PNPT1).

Application

Quality Control Testing
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PNPT1 in HeLa cell lysate.Tested applicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected PNPT1 in Jurkat, NIH3T3, and C6 cell lysates.Affinity Binding Assay: A representative lot of this antibody bound PNPT1 peptide with a KD of 2.5 x 10-8 in an affinity binding assay.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected PNPT1 in human stomach tissue sections.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-PNPT1, clone 2D23 ZooMAb, Cat. No. ZRB1066, is a recombinant Rabbit monoclonal antibody that specifically targets PNPT1 and has been tested for use in Affinity Binding Assay, Immunohistochemistry (Paraffin) and, Western Blotting.

Target description

Polyribonucleotide nucleotidyltransferase 1, mitochondrial (UniProt: A8TCS8; also known as EC:2.7.7.8, 3′-5′ RNA exonuclease OLD35, PNPase old-35, Polynucleotide phosphorylase 1, PNPase 1, Polynucleotide phosphorylase-like protein, PNPT1) is encoded by the PNPT1 (also known as PNPASE) gene (Gene ID: 87178) in human. PNPT1 is a peripheral homotrimer membrane protein that serves as a RNA-binding protein that is implicated in several RNA metabolic processes. It catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3′-to-5′ direction. It is synthesized with a transit peptide (aa 1-45) and is localized to mitochondrial intermembrane region. It is a component of the mitochondrial degradosome (mtEXO) complex with exoribonuclease activity that degrades 3′ overhang double-stranded RNA with a 3′-to-5′ directionality in an ATP-dependent manner. It is reported to regulate the expression of the electron transport chain components at the mRNA and protein levels. PNPT1 is shown to be essential for correct processing and polyadenylation of mitochondrial mRNAs and plays a role as a cytoplasmic RNA import factor that mediates the translocation of small RNA components into the mitochondrial matrix. It is also reported to participate in RNA cell surveillance by cleaning up oxidized RNAs. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service