Merck
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ZRB1108

Sigma-Aldrich

Anti-Catenin-α 2 Antibody, clone 3F7 ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

Synonym(s):
Alpha 2 Catenin, Alpha N-catenin, Alpha-catenin-related protein

biological source

rabbit

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

clone

recombinant monoclonal

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 105.31 kDa
observed mol wt ~110 kDa

species reactivity

rat, human, mouse

packaging

antibody small pack of 25 μL

greener alternative product characteristics

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enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 3F7 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Catenin-a2. It targets an epitope within 16 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal region of human Catenin-a2 .

Application

Quality Control Testing
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Catenin-a2 in Neuro-2A cell lysate.Tested applicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected Catenin-a2 in mouse and human brain lysates.Affinity Binding Assay: A representative lot of this antibody bound Catenin-a2 peptide with a KD of 2.8 x 10-7 in an affinity binding assay.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Catenin-a2 in HeLa cell line, HepG2 cell line, NIH 3T3 cell line and PC-12 cell line.Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Catenin- 2 in human cerebral cortex tissue sections.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Catenin- a2, clone 3F7 ZooMAb, Cat. No. ZRB1108, is a recombinant Rabbit monoclonal antibody that targets Catenin-a2 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Target description

Catenin-a2 (UniProt: P26232; also known as Alpha N-catenin, Alpha-catenin-related protein) is encoded by the CTNNA2 (also known as CAPR) gene (Gene ID: 1496) in Human. Catenins are a large family of cell-cell adhesion molecules that are involved in development and in epithelial cell polarity. They are reported to associate with both E- and N-cadherins. The association of catenins to cadherins creates a complex that is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Catenin- a2 is a peripheral membrane protein that is expressed in neural tissues. It is expressed in the developing cortical plate and marginal zone of 20-week-old human fetal brain. It functions as a linker between cadherin adhesion receptors and the cytoskeleton to regulate cell-cell adhesion and differentiation in the nervous system and is required for proper regulation of cortical neuronal migration and neurite growth. It is also reported to act as negative regulator of Arp2/3 complex activity and Arp2/3-mediated actin polymerization and hence suppresses excessive actin branching which would impair neurite growth and stability. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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