Merck
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ZRB1128

Sigma-Aldrich

Anti-PROX1 Antibody, clone 1B10 ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

Synonym(s):
Homeobox prospero-like protein PROX1, PROX-1, Prospero homeobox protein 1

biological source

rabbit

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

clone

recombinant monoclonal

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 83.2 kDa
observed mol wt ~83 kDa

species reactivity

monkey, human, mouse

species reactivity (predicted by homology)

rat

packaging

antibody small pack of 25 μL

greener alternative product characteristics

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enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 1B10 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Prospero homeobox protein 1 (PROX1). It targets an epitope within 15 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of human Prospero homeobox protein 1 (PROX1).

Application

Quality Control Testing
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PROX1 in SH-SY5Y cell lysate.Tested applicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected PROX1 in HepG2 cell lysate.Immunocytochemistry Analysis: A 1:1000 dilution from a representative lot detected PROX1 in SH-SY5Y cells.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected PROX1 in mouse brain tissue sections.Affinity Binding Assay: A representative lot of this antibody bound PROX1 peptide with a KD of 2.1 x 10-7 in an affinity binding assay.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-PROX1, clone 1B10 ZooMAb, Cat. No. ZRB1128, is a recombinant Rabbit monoclonal antibody that specifically targets PROX1 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Target description

Prospero homeobox protein 1 (UniProt: Q92786; also known as Homeobox prospero-like protein PROX1, PROX-1) is encoded by the PROX1 gene (Gene ID: 5629) in human. PROX1 is a transcription factor that is involved in multiple developmental processes including cell fate determination, gene transcriptional regulation and progenitor cell regulation in several organs. It plays a critical role in embryonic development and functions as a key regulatory protein in neurogenesis and the development of the heart, eye lens, liver, pancreas and the lymphatic system. It is most actively expressed in the developing lens and is also detected in embryonic brain, lung, liver and kidney. In adult, it is more abundant in heart and liver. PROX1 expression in the cardinal vein is shown to be essential for promoting and maintaining the budding of endothelial cells that eventually form the lymphatic vascular system. PROX1‐deficient mice are reported to lack lymphatic vasculature, and in these mice endothelial cells fail to acquire the lymphatic phenotype. PROX1 is also Involved in the regulation of the circadian rhythm. It represses transcription of the retinoid-related orphan receptor RORG, transcriptional activator activity of RORA and RORG and the expression of RORA/G-target genes including core clock components: ARNTL/BMAL1, NPAS2 and CRY1. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Hong, YK., et al. (2002). Dev. Dynamics. 225(3); 351-357).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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