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Anti-FAM49A Antibody, clone 2D6 ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

CYFIP-related Rac1 interactor A:CYRIA

biological source


Quality Level


expressed in HEK 293 cells



antibody form

purified antibody


recombinant monoclonal

product line

ZooMAb® learn more



mol wt

calculated mol wt 37.31 kDa
observed mol wt ~37 kDa

species reactivity


species reactivity (predicted by homology)

monkey, rat, mouse


antibody small pack of 25 μL

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enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation


affinity binding assay: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable



UniProt accession no.

greener alternative category

shipped in


storage temp.


General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.


Clone 2D6 is ZooMAb rabbit recombinant monoclonal antibody that specifically detects CYFIP-related Rac1 interactor A (FAM49A). It targets an epitope within 24 amino acids from the N-terminal region.


KLH-conjugated linear peptide corresponding to 24 amino acids from the N-terminal region of human CYFIP-related Rac1 interactor A (FAM49A).


Quality Control Testing
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected FAM49A in Human Hippocampus Membrane extractTested applicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected FAM49A in Human tonsil tissue lysate.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected FAM49A in U2OS cells.Flow Cytometry Analysis: 1 μg from a representative lot detected FAM49A in one million U2OS cells.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected FAM49A in human tonsil tissue sections.Affinity Binding Assay: A representative lot of this antibody bound FAM49A peptide with a KD of 5.8 x 10-7 in an affinity binding assay.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-FAM49A, clone 2D6 ZooMAb, Cat. No. ZRB1196, is a recombinant Rabbit monoclonal antibody that targets FAM49A and is tested in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Target description

CYFIP-related Rac1 interactor A (UniProt: Q9H0Q0; also known as Protein CYRIA, FAM49A) is encoded by the CYRIA (also known as FAM49A) gene (Gene ID: 81553) in human. FAM49A is a evolutionarily conserved protein that is suggested to negatively regulate RAC1 signaling and RAC1-driven cytoskeletal remodeling. It may also regulate chemotaxis, cell migration, and epithelial polarization by controlling the polarity, plasticity, duration and extent of protrusions. It binds to activated Rac1 via a domain of unknown function (DUF1394). This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Whitelaw, JA., et al. (2019). Commun. Integr. Biol. 12(1); 112-118).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.


30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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