Merck
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ZRB1626

Sigma-Aldrich

Anti-GABA A Receptor α1 Antibody, clone 3H10 ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

Synonym(s):
GABA(A) receptor subunit alpha-1, Gamma-aminobutyric acid receptor subunit alpha-1

biological source

rabbit

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

clone

recombinant monoclonal

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 51.8 kDa
observed mol wt ~51 kDa

species reactivity

mouse, human, rat

species reactivity (predicted by homology)

monkey, bovine

packaging

antibody small pack of 25 μL

greener alternative product characteristics

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enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

affinity binding assay: suitable
immunocytochemistry: suitable
western blot: suitable

isotype

IgG

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 3H10 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Gamma-aminobutyric acid receptor subunit a1

Immunogen

GST/His-tagged recombinant fragment corresponding to 125 amino acids from the N-terminal, extracellular domain of human GABA(A) receptor subunit a1 .

Application

Quality Control Testing
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GABA A Receptor 1 in SH-SY5Y cell lysate.Tested applicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected GABA A Receptor 1 in human cerebellum, mouse brain membrane, and rat brain tissue lysates.Affinity Binding Assay: A representative lot of this antibody bound GABA A Receptor a1 with a KD of 4.2 x 10-6 in an affinity binding assay.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GABA A Receptor 1 in PC12 and SH-SY5Y cells.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-GABA A Receptor a1, clone 3H10 ZooMAb, Cat. No. ZRB1626, is a recombinant Rabbit monoclonal antibody that specifically targets GABA A Receptor a1 and is tested for use in Affinity Binding Assay, Immunocytochemistry, and Western Blotting.

Target description

Gamma-aminobutyric acid receptor subunit a1 (UniProt: P14867; also known as GABA(A) receptor subunit alpha-1) is encoded by the GABRA1 gene (Gene ID: 2554) in human. GABA is the major inhibitory neurotransmitter in the central nervous system that inhibits the generation of the action potential of the neuron. It plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA-gated ion channel. It is involved in the pathogenesis of certain neurological and psychiatric disorders. GABA interacts with the GABAA and GABAB receptors, which are widely distributed throughout the nervous system and in a variety of cell types. On binding of two GABA molecules to the extracellular receptor domain, a conformational change is induced in the oligomer and Cl moves into the cell leading to an inhibitory hyperpolarization. GABAA and GABAB receptors differ in their pharmacological, electrophysiological, and biochemical properties. GABA a1 receptor subunit is part of the heteropentamer formed by a combination of a1, b2, and y2 subunits. GABA A receptor a1 subunit is synthesized with a signal peptide (aa 1-29) which is subsequently cleaved off to generate the mature form that contains four transmembrane domains (aa 252-273; 279-300; 313-334; and 422-443), one extracellular domain (aa 28-251), and a cytoplasmic domain (aa 335-421). This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Zhu, S., et al. (2018). Nature. 559(7712); 67-72; Goetz, T., et al. (2007). Prog. Brain Res. 160; 21-41).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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