Protein Purification Chromatography
For decades, liquid chromatography has been a powerful tool for isolating proteins, peptides, and other molecules from complex mixtures. Protein A, Protein G and Protein L bind IgG from a variety of species; resins crosslinked to these proteins are used to purify monoclonal or polyclonal antibodies. Affinity resins for fusion tags added to recombinant proteins allow purification by affinity chromatography. To support your protein research, we offer a broad range of protein chromatography tools for antibody purification and affinity purification, including the FLAG® system of recombinant fusion protein detection and purification.
FLAG purification
Expression vectors, affinity resins, and antibodies for expressing, purifying, and detecting FLAG® tagged fusion proteins.
Recombinant/Fusion Tag Protein Purification
Affinity purification resins and buffers for purifying His-tagged, GST-tagged, FLAG® tagged, S-tag™, Strep-Tag II, and T7-tagged proteins under native or denaturing conditions using batch or column purification techniques.
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Antibody purification resins and prepacked columns for lab scale, process scale, and industry scale workflows.
Streptavidin and avidin resins and prepacked columns for small- to large-scale purification of biotinylated proteins and antibodies.
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ANTI-FLAG® M2 antibody, Mouse monoclonal
1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)
ANTI-FLAG® M2 Affinity Gel
purified immunoglobulin, buffered aqueous glycerol solution
Anti-FLAG® M2 Magnetic Beads
affinity isolated antibody
ANTI-FLAG® M2 antibody, Mouse monoclonal
clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)
3xFLAG™ Peptide
≥90% (HPLC/MS), lyophilized powder
ANTI-FLAG® M2-Peroxidase (HRP) antibody, Mouse monoclonal
clone M2, purified immunoglobulin, buffered aqueous glycerol solution
ANTI-FLAG® antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
d-Desthiobiotin
≥98% (TLC)
FLAG® Peptide
≥85% (HPLC), lyophilized powder
PureProteome Protein A/G Mix Magnetic Beads
The PureProteome Protein A/G Mix Magnetic Bead System provides researchers with the power of the combination of both Protein A and Protein G immunoglobulin binding affinities in one convenient magnetic bead product.
Monoclonal Anti-HA−Agarose antibody produced in mouse
clone HA-7, purified immunoglobulin, PBS suspension
HIS-Select® Nickel Affinity Gel
(1:1 suspension in a 20% ethanol solution)
PureProteome Protein A Magnetic Bead System
The PureProteome Protein A Magnetic Bead System is a powerful system that helps researchers purify proteins by maximizing recovery and eliminating variability
EZview™ Red ANTI-FLAG® M2 Affinity Gel
clone M2
Protein G Agarose
>98% (HPLC and SDS-PAGE), suspension
Anti-HA Magnetic Beads
Magnetic agarose, suspension
ANTI-FLAG® M2-FITC antibody, Mouse monoclonal
clone M2, purified immunoglobulin, buffered aqueous solution
Glutathione−Agarose
lyophilized powder
Streptavidin−Agarose from Streptomyces avidinii
buffered aqueous suspension
EZview™ Red Anti-HA Affinity Gel
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