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DNA and RNA purification

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Reliable DNA extraction from Whatman™ FTA cards
This study examined the yield and quality of DNA from samples applied to Whatman FTA cards, using five common methods of DNA extraction.
Protector RNase Inhibitor Protocol & Troubleshooting
The addition of Protector RNase Inhibitor to cell pellets is not recommended. Protector RNase Inhibitor as a 50kDa protein will most probably not permeate into intact cells. If the cell pellet is immediately frozen at -80 °C, this will be
Saliva DNA Extraction & WGA Amplification Protocol
Whole Genome Amplification can be performed on DNA extracted in many ways. We offer many products for DNA extraction, including the GenElute™ Blood Genomic DNA Kit, GenElute Mammalian Genomic DNA Miniprep Kit and the GenElute Plant Genomic DNA M iniprep.
Plant DNA Extraction & WGA Amplification Protocol
GenomePlex® Whole Genome Amplification has been used to amplify genomic DNA from soybean, corn, tomato, purple coneflower, and ginseng.
Nucleic Acid Sample Preparation Methodology
Spectrophotometry can be used to estimate DNA or RNA concentration and to analyze the purity of the preparation.
Plant/Fungi Total RNA Purification Kit
The Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA from a wide range of plant and filamentous fungal species.
DNAgard® Blood Protocol
DNAgard Blood is designed for shipping and storage of DNA in mammalian whole blood. DNA in blood is protected by the unique stabilization properties of DNAgard Blood
Buccal DNA Extraction & WGA Amplification Protocol
This protocol provides a simple and convenient method to isolate, amplify, and purify genomic DNA from buccal swabs. Buccal swabs are a convenient method of acquiring a DNA sample.
DNAstable® Protocol
DNAstable is a unique storage medium that preserves genomic DNA, plasmids, bacterial artificial chromosomes (BACs), PCR products and oligonucleotides at room temperature.
Protein Sample Concentration
We provide gentle, membrane-based Protein Concentration options to concentrate proteins without compromising yield, even when labile or low in abundance.
TRI Reagent® Protocol
TRI Reagent is a single-step RNA isolation reagent that allows simultaneous isolation of DNA, RNA and protein. This protocol outlines sample prep as well as extraction of the various samples and troubleshooting.
SYBR® Green Extract-N-Amp™ Tissue PCR Kit Protocol
The SYBR® Green Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed for rapid extraction, amplification and detection of genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
Rapid Genotyping of Mouse Tissue Using Extract-N-Amp Tissue PCR Kit
Genomic detection of DNA via PCR amplification and detection on an electrophoretic gel is a standard way that the genotype of a tissue sample is determined. Conventional preparation of tissues for PCR-ready DNA often take several hours to days, depending
DNase I Recombinant, RNase-free Protocol
DNase I from bovine pancreas is a glycoprotein of Mr 37000. A special procedure is used to remove RNases from the DNase preparation.
Whole Genome Amplification: Extraction Protocol - Blood Card
Whole Genome Amplification: Extraction Protocol - Blood cards provide the convenience of archiving small volumes of blood. However, many times genomic DNA from these samples is limited, which may hinder the researcher’s ability to perform downstream analysis. This protocol provides
Extract-N-Amp™ Tissue PCR Protocol for Formalin-fixed Paraffin Embedded Sections
The following procedure is a modification of the Extract-N-Amp™ Tissue PCR protocol.
Oligonucleotide Handling & Stability
Handing and stability techniques to reduce variation and ensure trouble-free experiments.
Plasmid DNA Purification
Plasmids purified from genomic DNA, proteins, ribosomes, and the bacterial cell wall are used in molecular biology research. Various methods have been developed for plasmid DNA purification.
GenElute™ Mammalian Genomic DNA Miniprep Kit Protocol
The GenElute Mammalian Genomic DNA Purification Kit Protocol describes the isolation of pure, high molecular weight DNA from a variety of mammalian sources.
GenElute™ Bacterial Genomic DNA Kit Protocol (NA2100, NA2110, NA2120)
GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.
FFPE Tissue
Archived Formalin-fixed, Paraffin-embedded (FFPE) tissue samples are invaluable resources for profiling gene expression and studying a variety of diseases.
REDExtract-N-Amp™ Plant PCR Kit Protocol
The REDExtract-N-Amp Plant PCR kit includes an inert loading dye in the ReadyMix for easy gel loading after amplification. This protocol provides a rapid protocol to extract plant genomic DNA for amplification using the optimized ReadyMix.
Extract-N-Amp™ Tissue PCR Kit Protocol
The Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
Selecting Correctly Expressing Recombinants
Blue White Screening; DNA Minipreps; Screening by Restriction Digestion; Screening by PCR; Confirm cut plasmid sizes by agarose gel electrophoresis; DNA Maxipreps; DNA Precipitation; RNase Treatment; Clean-up of DNA
GenElute™ HP Plasmid Maxiprep Procedure
Follow this simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures with the GenElute™ HP Plasmid Maxiprep kit.
Viral RNA Purification Protocol Using GenElute™ Spin Prep Kits
Instructions for purifying viral RNA using GenElute™ RNA purification spin prep kits.
Extract-N-Amp™ Plant PCR Kits Protocol
The Extract-N-Amp™ Plant PCR Kits contain all of the reagents required to rapidly extract and amplify genomic DNA from plant leaves.
Biological Sample Collection, Transport, Archiving, and Purification for Nucleic Acid Analysis
A number of options are available for sample collection, transport, archiving, and DNA purification.
Plasmid DNA Preparation
Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.
Plasmid DNA Purification using illustra™ plasmidPrep Mini Spin Kit
This page shows Plasmid DNA purification using illustra™ plasmidPrep Mini Spin Kit from Cytiva.
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