Mammalian cell culture

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Cell Culture Protocol 5: Subculture of Suspension Cell Lines

Cell culture protocol for passaging and splitting suspension cell lines

Cell Analysis Best Practices

How do you assess the state of your cell culture? We’ve got some best practices to share.

F-12 Coon's Modification Formulation

McCoy's 5A Modified Media Formulation

3D Neurosphere Culture Media

3D neurosphere culture protocol to expand human U-87 MG glioblastoma cells in serum-free/xeno-free stem cell culture media. Explore over 350 PromoCell products on Sigma.com.

Linoleic acid in Cell Culture

Importance and uses of linoleic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

Common Cell Culture Problems: Precipitates

Common Cell Culture Problems: Precipitates -- When contamination is ruled out, turbidity in cell culture media is often explained by the precipitation of metals, proteins, and other media components. Precipitates can be harmful to cell health, as they may alter

Identification of Cell Culture Media with Raman Spectroscopy

Identification of cell culture media samples by traditional LC methods, such as amino acid or vitamin analysis, has high costs, and requires significant analytical expertise and laboratory space.

Cultivating Consistency: Tips and Tricks for Successful Cell Cultures

Properly optimizing workflows and utilizing the latest best practices are the keys to obtaining consistent results and advancing research.

Autophagy in Cancer Promotes Therapeutic Resistance

We presents an article on Autophagy in Cancer Promotes Therapeutic Resistance

Fibroblast Growth Factor Family (FGF)

Fibroblast growth factors in cell culture and various growth factors for your research

Insulin-like Growth Factors (IGF)

Hematopoietic Cytokines

Read article on hematopoietic cytokines and hematopoiesis

Cell Freezing and Banking

Removal of Percoll after Centrifugation

This page shows methods to remove Percoll from a sample after centrifugation.

Human Hematopoietic Progenitor Cells (HPC) and Growth Media

CD34+ Progenitor Cells are suitable for diverse studies, as well as for the directed differentiation into more committed blood cell phenotypes and endothelial lineages.

L-Ornithine in Cell Culture

Discover L-ornithine, an important amino acid supplement, explore how L-ornithine is used in CHO and iPSC culture, and discover how poly-L-ornithine is used to enhance cell attachment.

L-Tyrosine in Cell Culture

Learn more about the amino acid L-Tyrosine and explore how it is used in cell culture media like MEM, DMEM, and RPMI. We offer a comprehensive portfolio of amino acid cell culture supplements and reagents.

β-Alanine in Cell Culture

Learn more about β-Alanine, an important amino acid supplement and explore how it is used in cell culture. We offer a comprehensive portfolio of amino acid cell culture supplements and reagents.

Heparin Versus Heparin Sulfate Proteoglycans

Heparin is distinct from heparan sulfate proteoglycans (HS) which is primarily produced by mast cells. While, HS is produced by all cell types. Know more about the difference between heparin and heparin sulfate proteoglycan and select the right component for

DMEM/nutrient Mixture F-12 Formulation

Dulbecco's Modified Eagle's Medium/Ham's Nutrient Mixture F-12 (DME/F12) Formulation

Cell Line Cross-Contamination and Misidentification; Common Cell Culture Problems

Cell line cross-contamination and misidentification can be solved with authentication and conscientious sourcing.

Fetal Bovine Serum (FBS) – What You Need to Know

Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.

Glucose in Cell Culture

Importance and uses of glucose in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

Ascorbate in Cell Culture

Importance and uses of ascorbate in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

Biotin in Cell Culture

Importance and uses of biotin in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

Ferric and Ferrous Iron in Cell Culture

Importance and uses of ferric and ferrous iron in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

Vitamin E, Tocopherol, in Cell Culture

Importance and uses of vitamin E (tocopherol) in serum-free eukaryotic, including hybridoma and chinese hamster ovary (CHO), cell cultures.

Serum for Cell and Tissue Culture FAQs

Find answers to frequently asked questions (FAQs) about FBS and other serum products, and links to related products.

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