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Restorase® DNA Polymerase
Restorase® was developed for researchers unable to achieve amplification of damaged DNA templates when using other commercially available DNA polymerases.
Polymerase Chain Reaction
The polymerase chain reaction is one of the most widely used techniques in molecular biology. The PCR process consists of three main steps, Denaturation, Annealing & Extension
PCR Assay Optimization and Validation
PCR assay guide navigates you through primer validation and other assay optimization factors to ensure high sensitivity and specificity for optimum DNA/ RNA quantification.
ThermaGenix PCR Additives Reduce Background and Increase Yield
How to stop nonspecific bands and off target primers in PCR.
ThermaStop™-RT PCR Additive
PCR additive interacts with reverse transcriptase at low temperatures to reduce priming problems that lead to nonspecific bands.
Optimizing Crude Plant Samples for PCR
Overcoming potent inhibitors and sample diversity in direct plant PCR.
Whole Transcriptome Amplification of RNA from Low Cell-Number Samples
The efficacy of amplification of small quantities of total RNA with the Complete Whole Transcriptome Amplification Kit (WTA2) was examined in this study.
FastStart™ Taq DNA Polymerase, 5 U/μL Protocol & Troubleshooting
The choice of the PCR enzyme in combination with an appropriate buffer can profoundly affect PCR outcome.
5'/3' RACE Kit, 2nd Generation Troubleshooting
In addition to the troubleshooting provided in the product manual, most probably the efficiency of the tailing reaction performed by Terminal Transferase could be impaired. This could occur due to several reasons (which will not only affect the control reaction
Complete Solutions for PCR Assay Development
Fit-for-use products offer the quality, consistency & documentation necessary for every step of your IVD development and manufacturing process.
Extract-N-Amp™ Product Guide
Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.
Oligonucleotide Quality Control & Quality Assurance
The foundation of Sigma's manufacturing process is a robust QMS, which drives compliance to ISO 9001:2008 certification.
Custom LAMP Primers in Partnership with New England Biolabs
Primers manufactured using proprietary purification adjustments to perform optimally in loop-mediated isothermal amplification assays.
KAPA3G Plant PCR Kits FAQs
Amplify up to 5 kb fragments from purified plant DNA extracted using commercial kits or CTAB-based methods. Review some of the most asked questions regarding direct PCR from leaf disks, seeds and other plant tissues.
KAPA Express Extract Kit FAQs
Extract DNA from crude samples for PCR applications, including qPCR, using the KAPA Express Extract Kit. See frequently asked questions regarding sample type, optimization and storage.
GC-RICH PCR System Troubleshooting
GC-RICH Amplification of Polymerase Chain Reaction (PCR) System Troubleshooting.
Extract-N-Amp™ Blood PCR Kit Protocol
The Extract-N-Amp Blood PCR Kits contain all the reagents needed to rapidly extract and amplify human genomic DNA from whole blood, whole blood dried on a blood card, and cultured mammalian cells.
RT-PCR Troubleshooting
PCR troubleshooting guide for reliable data in molecular assays.
PCR/qPCR/dPCR Assay Design
PCR workflow's variability influenced by sample source, reverse transcription, and assay design, impacting reproducibility.
MIQE: Real-Time PCR Experiment Standards
MIQE-compliant qPCR services support primer design, protocol development, and data analysis for faster product development and publication.
Array CGH Analysis with Whole Genome Amplification
aCGH technology evolves for high-res chromosomal analysis, needing a large DNA input per sample, advancing genetic research.
PCR Troubleshooting Tips
PCR troubleshooting protocols require exploring all potential error sources to ensure reliable results, addressing each problem independently.
Extract-N-Amp™ Plant PCR Kits
Extract-N-Amp kits extract and amplify genomic DNA, optimized for plant tissue without inhibition concerns.
Whole Genome Amplification for Single Cell Biology
Whole genome amplification overcomes restrictions for single-cell genomic analyses with non-specific amplification.
WTA2 Amplicon Modification for NGS
Transplex Whole Transcriptome Amplification (WTA2) precisely amplifies RNA maintaining transcript levels in test and reference samples.
DNA Oligonucleotide Synthesis
Learn about the steps in phosphoramidite solid-phase method of Oligonucleotide Synthesis.
Locked Nucleic Acid & Minor Groove Binder / Eclipse Dark Quencher
Explore Locked Nucleic Acid and MGB. These oligo modifications offer stability and specificity in molecular analysis, making them ideal for research and diagnostic applications.
Oligonucleotide Quantification
DNA can be easily quantitated in a UV spectrometer due to its highly conjugated nature.
Nuclease-Free Water at Your Fingertips
An ultrafiltration cartridge can be placed at the outlet of a water purification system to deliver nuclease-free ultrapure water.
Oligonucleotide Quality Control by Mass Spectrometry
Mass Spectrometry is the technology of choice for analyzing oligonucleotide synthesis. It enables the most sensitive detection of low levels of by-products, which can affect performance.
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