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Use of PEPscreen® Peptides to Investigate CTL Class I MHC and Th Class II Responses to AAV-hF.IX Gene Therapy
Recombinant adeno-associated virus (AAV)-mediated gene therapy expressing human coagulant factor IX (hF.IX) is a promising gene transfer strategy due to the long-term expression of hF.IX in the liver and weak immune response using a liver-specific promoter. However, in humans and
Duolink® Proximity Ligation Assay Instructional Video
This video will show you step-by-step how to perform a Duolink® PLA experiment with cells on a slide that have already been fixed and permeabilized by the user.
Use of PEPscreen® Libraries to Identify CD8 Epitopes Presented During Malaria Infection
Malaria caused by Plasmodium species kills more than one million people per year, 90% of them in sub-Saharan Africa. Our previous work had shown that CD8 T cells are a major component of immunity to the first stage of infection
Use of PEPscreen® Peptides to Investigate Protein Farnesyltransferase Substrate Specificity
Prenylation is an essential post-translational modification for the proper localization and function of many proteins and is catalyzed by protein farnesyltransferase (FTase) and protein geranylgeranyltransferase type I (GGTase-I). To identify potential novel prenylated proteins, we used a PEPscreen peptide library
Sulfoxide-Containing Mass Spectrometry (MS)-Cleavable Cross-Linkers
Learn about homobifunctional sulfoxide-containing MS-cleavable cross-linkers that enables the capture of protein-protein interactions (PPIs) in native cellular environments, and the identification of cross-linked peptides permits the determination of both identity and connectivity of PPIs.
Duolink® Proximity Ligation Assay (PLA) Multicolor Kits for Sensitive Multiplex Detection
Duolink® PLA Multicolor technology and the quantification of protein-protein interactions in fixed tissues and cells.
RNA Immunoprecipitation Chip (RIP) Assay
RNA Immunoprecipitation (RIP) is an essential method for analyzing proteins that interact with and modify the function of mRNAs, small RNAs, viral RNAs, or lncRNAs.
PEPscreen® Dissolution, Storage and Handling Guidelines
In order to obtain accurate and reliable peptide activity data, careful attention should be devoted to the process of dissolving peptide library sets.
FLAG® HA System
The FLAG® HA tandem epitope system consists of small epitopes tags that are not eukaryotic derived. These features minimize interference with protein functions and provide superior specificity.
Fragmentation of chromatin in ChIP
Fragmentation of chromatin in Chromatin Immunoprecipitation (ChIP)
Guides to cell number for ChIP and endpoint analysis
Guides to cell number for Chromatin Immunoprecipitation (ChIP) and endpoint analysis
ChIP: Immunoprecipitation, washing and elution
Chromatin Immunoprecipitation (ChIP), washing and elution
Chromatin Immunocleavage (ChIC) kits and CUT&RUN technology overview for improved chromatin isolation and downstream analyses.
ChIC and CUT&RUN Data Analysis Tool Overview
Chromatin Immunocleavage (ChIC) and CUT&RUN data analysis tool for file management and downstream sequencing data analyses.
How to Optimize the Duolink® Proximity Ligation Assay
Things to consider for preparation, setup and execution of the Duolink® assay protocol
PEPscreen® Peptide Library Stability
Peptide stability is impacted by several factors including temperature, moisture content, amino acid composition and sequence. PEPscreen peptides are manufactured and shipped to ensure optimal shelf life.
Use of Peptide Libraries to Investigate the Evolutionary Preservation of Antimicrobial Properties of Complement Peptide C3a from Invertebrates to Humans
This study used a combination of analyses to identify the structural pre-requisites governing antimicrobial activity of C3a from humans back to prehistoric invertebrates.
Detection of Protein-protein Interactions by in situ PLA
Proteins are complex biological molecules essential for cellular structure and functions. The majority of proteins commonly interact with various molecules, including other proteins, in order to exert their functions.
How Proximity Ligation Assays (PLA) Work
Learn how Proximity Ligation Assay technology works and how the protein-protein interaction control kit can confirm in situ detection of EGF-induced EGFR-HER2 dimerization.
ChIP-X: Cross-linking proteins to DNA and cell lysis
X-ChIP: Cross-linking proteins to DNA and cell lysis
Studies of Dimerization and Activation of Receptors in the EGFR-Family Using Duolink® Assays
Our aim was to investigate the dimerization and activation pattern of two receptors, EGFR (ErbB1) and HER2 (ErbB2), in the EGFR-family using breast cancer cell lines and selected colon tissues as model systems.
How to Optimize Duolink® PLA for Flow Cytometry Detection
Considerations for proper experimental design, preparation and execution of the Duolink® PLA for flow cytometry protocol.
PEPscreen® Peptide Library Frequently Asked Questions (FAQ)
PEPscreen peptides are synthesized on proprietary, state-of-the-art robotic platforms utilizing optimized protocols based on Fmoc-chemistry.
Duolink® PLA Technology for Neuroscience Applications
Duolink® proximity ligation assay used to study neuron interactions furthering neuroscience research.
RNA Immunoprecipitation (RIP) FAQs
RNA Immunoprecipitation (RIP) Frequently Asked Questions
ChIP Troubleshooting
Frequently ask questions about ChIP.
Duolink® References
Find Duolink references based on the type of method used, post translational modification detected, and research focus.
ChIP Troubleshooting Tips
Chromatin Immunoprecipitation (ChIP) Troubleshooting Tips
Tips for Optimizing Immunofluorescence Protocols to Get the Best Image
Uses for immunofluorescence (IF)—where an antibody conjugated to a molecule that fluoresces when excited by lasers— include protein localization, confirmation of post-translational modification or activation, and proximity to/complexing with other proteins.
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