Protein expression

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Antioxidants protect biological systems from oxidative damage produced by oxygen-containing free radicals and from redoxactive transition metal ions such as iron, copper, and cadmium.

Understanding Sweet Taste Perception

The powerful effect of the pleasurable sensation arising from sweet tasting foods has led to consumer demand and large-scale production of the caloric sweetening agents sucrose and fructose.

Troubleshooting in pGEX Expression Vectors

This page describes troubleshooting strategies for cloning the gene or gene fragment into a pGEX expression vector.

Duolink® Proximity Ligation Assay Instructional Video

This video will show you step-by-step how to perform a Duolink® PLA experiment with cells on a slide that have already been fixed and permeabilized by the user.

GABAB Receptors

We offer many products related to GABAB receptors for your research needs.

Duolink® Proximity Ligation Assay (PLA) Multicolor Kits for Sensitive Multiplex Detection

Duolink® PLA Multicolor technology and the quantification of protein-protein interactions in fixed tissues and cells.

How to Optimize the Duolink® Proximity Ligation Assay

Things to consider for preparation, setup and execution of the Duolink® assay protocol

Sensor Dyes

Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.

Signal Peptide Optimization: Effect On Recombinant Monoclonal IgG Productivity, Product Quality And Antigen-Binding Affinity

A signal peptide is a 5-30 amino acid (aa) peptide present at the N-terminus of secretory proteins.

Protein-based Drug Transport

Protein-based drug transporters are expressed in Sf9 cells. Understanding the specific mechanisms of tumor cell transporters is an essential aspect of chemotherapeutic drug design.

Epidermal Growth Factor Receptor (EGFR) Signaling

Review EGF/EGFR signaling (epidermal growth factor receptor) and find links to related products.

Caspases Inhibitors and Activators

Agents reported to activate cellular caspases include chemotherapeutic drugs, TNF receptor agonists, and other enzymes. Inhibitors of apoptosis were the first identified endogenous caspase inhibitors.

Chloride Channels

We offer many products related to chloride channels for your research needs.

Cyclin-dependent Kinases (CDKs)

Review properties, activators and inhibitors, and available products for researching cyclin-dependent kinases (CDKs).

Focal Adhesion Kinase (FAK) Overview

The focal adhesion kinase (FAK) is a cytoplasmic protein tyrosine kinase. FAK has been implicated as a downstream signaling molecule that functions in the control of several integrin-regulated biological processes.

Calcium Channels

We offers many products related to calcium channels for your research needs.

Somatostatin Receptors

Somatostatin (somatotropin release-inhibiting factor, SRIF) is a cyclic peptide widely expressed throughout the CNS, endocrine tissues, and gastrointestinal tract.

Vasopressin and Oxytocin Receptors

Sigma-Aldrich offers many products related to vasopressin and oxytocin receptors for your research needs.

Nuclear Receptors (Non-Steroids)

We offer many products related to non-steroid nuclear receptors for your research needs.

Histamine Synthesis and Metabolism

Our company offers many products related to histamine synthesis and metabolism for your research needs.

β-Adrenoceptors

β-Adrenoceptors are activated either by norepinephrine or epinephrine. Learn more about the role of β-adrenoceptors in protein expression, cell signaling, and metabolism research.

GABAC Receptors

Introduction to GABAC receptors as well as modulators and other products for your research needs.

Glutamate Receptors (G Protein Family)

Sigma-Aldrich offers many products related to G-protein family glutamate receptors for your research needs.

α2-Adrenoceptors

Learn about alpha-2 adrenoceptor and its subtypes, mediated responses, and applications of agonists. Included is a list of available products and a comparison table.

GABAA Receptors

We offer many products related to GABAA receptors for your research needs.

Dopamine, Norepinephrine and Ephinephrine Synthesis

Dopamine-β-hydroxylase is located inside amine storage vesicles of norepinephrine neurons. Dopamine is actively transported from the cytoplasm into the vesicles. As the enzyme is a copper containing protein, its activity can be inhibited by copper chelating agents, such as diethyldithiocarbamate

Canonical Wnt / β-Catenin Signaling

We review the Wnt signaling pathway (also, canonical Wnt or beta (β)-catenin pathway) and provide links to related products at sigmaaldrich.com.

Inhibition of Protein Synthesis by Antibiotics

Antibiotics targeting bacterial ribosomes disrupt protein synthesis, a key process in bacterial growth inhibition.

Glutamate/GABA Synthesis & Metabolism

Glutamine's role in neurotransmitter synthesis and transport highlights its importance in neuronal function and glutamate production.

Glutamine Metabolism in Cancer Cells

Sigma article discusses tumor cell metabolic pathways, focusing on aerobic glycolysis and mitochondrial activity.

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