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Protein labeling and modification
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Self-Assembled Monolayers: Advantages of Pure Alkanethiols
We presents an article regarding Self-Assembled Monolayers: Advantages of Pure Alkanethiols.
Ni-NTA-Atto Conjugates
Ni-NTA-Atto conjugates provide specific and highly sensitive detection of His-tagged fusion proteins.
Near-infrared (NIR) Fluorescence Dyes
NIR Fluorescence Dyes
Enzymes for Antibody Characterization
Antibodies now make up the fastest growing category of therapeutic drugs
Chymotrypsin
Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.
Cleavage and Purification of GST-Tagged Protein Bound to Glutathione Sepharose in Batch Mode
This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification
Peptide Modifications: N-Terminal, Internal, and C-Terminal
Peptide Modifications: N-Terminal, Internal, and C-Terminal
Introduction to Target Identification with Hyas and Dione Kits
In cases where photoaffinity labeling (PAL) probes are ineffective, Hyas and Dione Kits provide an effective method for small molecule identification by using an Iridium photocatalyst tethered to the molecule under investigation.
Signal Peptide Optimization: Effect On Recombinant Monoclonal IgG Productivity, Product Quality And Antigen-Binding Affinity
A signal peptide is a 5-30 amino acid (aa) peptide present at the N-terminus of secretory proteins.
Proline Derivatives and Analogs
Proline analogues are promising candidates for tuning the biological, pharmaceutical, or physicochemical properties of naturally occuring, as well as de novo designed, linear, and, cyclic peptides.
Review of O-Linked Glycoproteins
O-Linked glycoproteins are usually large proteins with a molecular mass of >200 kDa. Glycosylation generally occurs in high-density clusters and may represent as much as 50-80% of the overall mass.
Glycosylphosphatidylinisotol-anchored Glycoproteins Overview
GPI Anchored Glycoproteins
Protein Deglycosylation
Protein Deglycosylation: PNGase F Proteomics Grade, Native Protein Deglycosylation Kit, Enzymatic Protein Deglycosylation Kit, and ProteoProfile Trypsin In-Gel Digest Kit
Improving Glycoform Coverage for Mucin Prep
Human Protein Atlas uses A-431, U-251 MG, and U-205 cell lines with Prestige Antibodies for organelle mapping.
Biotinylated Peptides
Streptavidin HC plates with high-capacity coating offer selective binding, reducing non-specificity.
GlycoProfile™ ß-Elimination Kit
Our GlycoProfile Beta-Elimination Kit enables comprehensive O-glycoproteomics research, overcoming challenges in complete O-deglycosylation.
Strategies for Deglycosylating N-Linked Glycans
Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.
Solid Phase Synthesis
Peptides synthesized using FMOC or BOC chemistry on PEG-Polystyrene support resin, then cleaved, precipitated, and lyophilized.
Introduction to Proximity Labeling
Novel method tags antibodies with photoreactive iridium for membrane protein labeling.
Atto & Tracy Dyes for Protein Labeling
Protein labeling kits with Atto and Tracy dyes provide easy fluorescent labeling of purified proteins, enzymes, and antibodies.
Y-Shape Peg Derivatives in Drug Delivery
Immune system defends against disease by hydrolyzing foreign molecules; proteases, nucleases, liver, and kidneys are involved.
Peptide Coupling Reagents Guide
Novabiochem® coupling reagents for in situ activation are fast-reacting and compatible with various amino acids.
Mass Spectrometry
What is Mass Spectrometry or MS? What is it used for and how does it work? Read this detailed article with diagrams and example graphs to understand how to use mass spec in your research.
EX-CELL® Glycosylation Adjust (GAL+)
Our protein quality supplement, EX-CELL® Glycosylation Adjust (Gal+), provides customers with a novel chemically defined product which targets glycosylation attributes.
Utilizing ISOGRO® Supplementation of M9 Minimal Media to Enhance Recombinant Protein Expression
Utilizing ISOGRO® Supplementation of M9 Minimal Media to Enhance Recombinant Protein Expression.
ChemMatrix® Resin for solid phase peptide synthesis
ChemMatrix® is a proprietary, 100% PEG (polyethylene glycol) based resin from PCAS BioMatrix.
Troubleshooting Strategies in GST-tagged Protein Purification
This page describes troubleshooting strategies in purification of GST-tagged proteins using Cytiva products.
Chiral Amines in Asymmetric Synthesis
Chiral amines have found widespread application in asymmetric synthesis serving, for instance, as chiral bases in enantioselective deprotonation reactions or being valuable substances for resolving racemic mixtures of acids.
ChiPros Chiral Amines
Chiral amines play an important role in stereoselective organic synthesis. They are used directly as resolving agents, building blocks, or chiral auxiliaries.
Cleavage and Purification of GST-Tagged Protein Bound to GSTrap
This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.
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