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Hydrophilic Interaction Liquid Chromatography
An introduction to the HILIC separation technique and an overview of the strategies for developing robust methods for separation of polar and hydrophilic compounds.
BIOshell™ Glycan U/HPLC Columns
The BIOshell™ Glycan column is ideal for the separation and quantitation of isolated glycans using the HILIC method for accurate glycoprotein analysis.
Bioanalysis with SPME
Biocompatible SPME devices employ C18 bonded porous silica sorbent particles, in a binder which resists fouling by biological matrix components
Supel™-Select Polymeric SPE
Supel-Select HLB SPE - a hydrophilic modified styrene based polymer developed for the solid phase extraction of a broad range of compounds from aqueous samples.
Two Distinct Sample Prep Approaches to Overcome Matrix Effect in LC/MS of Serum or Plasma Samples
This article highlights the impact that sample matrix effects can have on LC/MS response and discusses two novel approaches to reduce it.
Rapid Determination of Protein Binding Affinity Using Solid Phase Microextraction
Accurate protein binding affinities were measured using bioSPME more rapidly than membrane dialysis (RED) techniques. Binding affinities correlated to the reference values.
Synthetic Cannabinoid Analysis
A large variety of cannabinoid drug standards, along with an HPLC method with the Supelco Ascentis® Express RP-Amide column, are presented in this article.
Improving the Chromatographic Separation of DMB-Labeled Sialic Acids for the Comparison of Biosimilars to Reference Materials
A significantly improved HPLC-fluorescence method for DMB-NANA and -NGNA, and application of this method to compare 2 candidate biosimilar therapeutic proteins to their respective RMs.
Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine
β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.
How to Extend Liquid Chromatography Column Life: Regenerate or Use Guard Columns
What is causing aging of a column? Is the regeneration/washing of the column a reasonable approach to extend the lifetime of a column? What are the risks & challenges? Learn more in this article.
HPLC Analysis of Active Cannabinoid Compounds in Gummy Bear Candy on Ascentis® Express Biphenyl with UV Detection after Extraction
Tetrahydrocannabinolic acid A solution, 1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material.
Improving the Current USP Method for the Analysis of Lansoprazole Drug Substance Using HPLC Columns Based on Fused-Core® Particle Technology
Compendial methods from the USP (United States Pharmacopeia) are widely used in pharmaceutical drug product and raw materials testing. However, not all methods in the USP use modern technologies. In chromatographic methods, it is not uncommon that older brands of
Ascentis® Express RP-Amide Columns for HPLC and UHPLC
Improve your separation of basic compounds with our Ascentis® Express RP-Amide HPLC columns from the Supelco® portfolio.
Ascentis® Express RP-Amide HPLC Columns
Sigma-Aldrich.com presents an article about innovative particle technology and RP-Amide bonding chemistry provide the benefits of speed, resolution, selectivity, aqueous stability, and improved peak shape for bases.
Method Optimization for LC-MS Analysis of Vitamin D Metabolite Critical Pairs in Serum
Using HybridSPE-Phospholipid, Ascentis Express, and Other LC-MS Workflow Components
Quick, Sensitive LC/MS/MS Analysis of Aflatoxins in Cannabis
Analyte recovery values from spiked cannabis extracts ranged from 102 to 127% with RSD below 12% for 3 replicates. Excellent recovery values were even observed for Aflatoxins B2 and G2 which were spiked at a low level of 6.1 ppb.
HPLC Tips & Tricks: Mobile Phase Preparation Part 2 - Buffers
Learn the tips and tricks of selecting and preparing a suitable mobile phase buffer for your HPLC separations and method developments for samples that contain ionizable compounds.
Simplified LC-MS/MS Method for Glyphosate, AMPA, and Glufosinate in Oat-Based Cereals
A simplified LC-MS/MS method to determine glyphosate and related compounds using a Supel™ Carbon LC U/HPLC column for stability under higher pH conditions and sufficient retention for the polar analytes in the presence of methanol as an extraction solvent.
HPLC Analysis of Xanthines on Purospher® STAR RP-18 endcapped
Xanthine is a purine base found in most human body tissues and fluids as well as in other organisms. Methylated xanthines (methylxanthines), which include caffeine, paraxanthine, theobromine, and theophylline, commonly used for their effects as mild stiµlants and as bronchodilators
Improved Analysis of Simple Sugars Using apHera™ NH2 HPLC Columns
The apHera NH2 polymeric column is an excellent choice for the separation of closely-related polar analytes such as simple sugars using HILIC chromatography.
HPLC Products: The Best Choice for Any LC Instrument
Discover our comprehensive portfolio of High-performance liquid chromatography (HPLC) columns and consumables for your various and specialized U/HPLC based applications― 100% compatible with HPLC, UHPLC, or UPLC® systems from any vendor.
Determination of Tramadol in Urine using LC-MS/MS
This article presents an LC-MS/MS method for the determination of tramadol in urine samples using reversed-phase LC-MS/MS aided by isotope internal standards.
Cannabinoid Certified Reference Materials (CRMs) for an Improved Testing Accuracy and Traceability
We offer a portfolio of cannabinoid CRMs such as THC and THCA, available as single component solutions or mixes, certified in accordance with ISO 17034 and ISO/IEC 17025 for accurate cannabinoid profiling and potency testing.
Applications of Newly Developed 2.7 μm Porous Graphitic Carbon Particles in U/HPLC
Polar graphitic carbon (2.7 μm) based Ultra/High-performance liquid chromatographic separation of four highly polar pesticides, two polar herbicides―paraquat & diquat, and Vitamin D and its metabolites.
Determination of Formaldehyde and Acetaldehyde in Air Using DNPH Cartridges and Automated On-Line Desorption Followed by HPLC
Carbonyl compounds react within the DNPH cartridge to form immobilized hydrazones, which can be subjected to automated desorption and HPLC analysis with UV detection.
HPLC Tips & Tricks: Increase Your HPLC/UHPLC Method Sensitivity
Tips & tricks to improve your chromatographic method sensitivity by reducing baseline noise and/or making suggested improvements to increase signal intensity.
Ion Chromatography & Cation Suppression Straightforward Cation Analysis at Trace Levels
Ammonium determination in acidic solutions with Metrosep C Supp 2 column using the acidic binary IC eluent nitric acid/rubidium nitrate concentrate and the sodium bicarbonate/sodium carbonate suppressor regenerant.
Separation of Organic Acids with 100% aqueous mobile phase using an Ascentis® Express AQ-C18 HPLC Column
A new RP-HPLC method is proposed for the determination of organic acids in 100% aqueous mobile phase using an Ascentis® Express AQ-C18 column
LC-MS Analysis of Hormones in Tap Water
Milli-Q® ultrapure water systems deliver water free of organic contaminants , including hormones present in a lab's tap water, making it suitable for the most sensitive LC-MS/MS analyses.
Analysis of Pesticides in Turmeric Powder by LC/MS/MS and GC/MS/MS after Cleanup with Dual-Layer SPE Cartridge
Pesticide residue testing of turmeric and other (dried) spices is challenging due to their complex compositions and high matrix. An efficient SPE clean-up can be achieved by a new Dual-layer cartridge.
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