Search Within
Applications
Content Type
Topic
qPCR
Applied Filters:
facet applications:qPCR
facet content type:Protocol
Showing 1-28 of 28 results
SYBR® Green JumpStart™ Taq ReadyMix™
Protocol describes amplification of DNA through quantitative PCR with SYBR Green. Consistent batch-to-batch performance can be achieved with large numbers of PCR reactions.
Multiplex Real-Time PCR
Multiplex qPCR employing probe-based chemistries is a demanding application that often requires extensive optimization and validation.
Universal SYBR Green qPCR Protocol
Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions
qPCR Reference Gene Selection Protocol
Analysis of gene expression data requires a stable reference or loading control. This reference is usually one or more reference genes.
Protocol for Conjugating NHS-Ester Modifications to Amino-Labeled Oligonucleotides
Protocol for conjugating NHS-ester modifications to an oligonucleotide with an amino label.
FFPE Tissue
Archived Formalin-fixed, Paraffin-embedded (FFPE) tissue samples are invaluable resources for profiling gene expression and studying a variety of diseases.
KiCqStart™ One-Step Probe RT-qPCR
KiCqStart One-Step Probe RT-qPCR ReadyMix streamlines RNA template RT-qPCR with high sensitivity.
ChIP-qPCR Data Analysis
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
SPUD Assay for Detection of Assay Inhibitors Protocol
SPUD assay identifies inhibitors in RNA or DNA samples, useful for analyzing numerous or low-copy targets.
SYBR® Green Extract-N-Amp™ PCR Kit
SYBR® Green Extract-N-Amp™ Tissue PCR Kit enables rapid genomic DNA extraction and amplification from animal tissues.
qPCR Gene Expression Protocol Using SYBR Green
SYBR Green I dye in qPCR measures target quantity, adaptable to specific needs with a standard protocol.
qPCR with Single Detection Probe
qPCR protocol template with specific detection probes aids in single-target detection using LuminoCt® ReadyMix™.
Primer Concentration Optimization
Primer Concentration Optimization Protocol creates reaction matrix for testing primer concentrations against various partners.
Primer Optimization Using Temperature Gradient
Gradient PCR optimizes assay conditions by testing fixed primer concentrations across various annealing temperatures.
qPCR Efficiency Determination Protocol
Optimization of qPCR conditions is important for the development of a robust assay. The two main approaches are optimization of primer concentration and/or annealing temperatures.
ABI3900 Synthesizer Columns with CUTAG CPG Frits
Next generation CPG Frit support for reliable, cost-effective oligo synthesis from Proligo®, for ABI 3900 and MerMade synthesizers
Rapid SYBR® Green qPCR on the Illumina Eco™ Real-Time PCR System
Fast real-time PCR is a demanding application that requires consistent and reproducible results from the difficult amplicons and low-volume reactions
High-throughput Real-Time PCR: SYBR® Green
High-throughput qPCR using SYBR® Green is a demanding application that requires consistent and reproducible results from low-volume, high-speed assays.
RT-qPCR Basic Troubleshooting
Learn the basics of real-time PCR and qPCR with tips, tricks and general troubleshooting.
RNA Immunoprecipitation qPCR (RIP- qPCR) Protocol
RNA immunoprecipitation (RIP) can identify specific RNA molecules of many types associated with specific nuclear or cytoplasmic binding proteins.
KiCqStart™ Universal SYBR® Green qPCR Protocol
Quantitative PCR protocol using SYBR Green reagents. Procedure supports most qPCR instruments.
KiCqStart™ Probe qPCR ReadyMix™
KiCqStart™ Probe qPCR ReadyMix™ is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments.
SYBR® Green I Dye Quantitative PCR Protocol
A protocol that can be used as a basic template for qPCR incorporating SYBR Green I DNA binding dye that is amenable to modification and applicable for use as validation for a set of primers.
KAPA SYBR® FAST One-Step qRT-PCR Kits FAQs
Frequently asked questions (FAQs) for KAPA SYBR® FAST One-Step qRT-PCR Kits.
Multiplex qPCR Protocol
Standard qPCR protocol with up to four detection probes at specified concentrations, simplifying reaction setup with LuminoCt ReadyMix.
dPCR Protocol
Bio-Rad's droplet system partitions samples into 20,000 reactions per well, totaling 1.9 million reactions per plate.
The 3'/5' Assay for Analysis of RNA Integrity Protocol
The 3’/5’ integrity assay identifies RNA degradation, crucial for large sample sets or less detectable degradation.
Reverse Transcription Protocol (One-step Probe Detection)
Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.