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Preparing Cells for Electroporation
Preparing Cells for Electroporation
LC-UV-MS Method Development for Antibody Drug Conjugates Using a Non-Toxic ADC-Mimic
SigmaMab Antibody Drug Conjugate Mimic, is a non-toxic drug mimic utilized as a standard for mass spectrometry and high performance liquid chromatography.
Rapid DNA Ligation Kit Protocol
Rapid DNA Ligation Kit Protocol
DNA Ligation Protocol
The cloning process requires the ligation of linear DNA into a cloning vector. This ability to join fragments of DNA through recombinant technology is essential for many basic experiments in biotechnology.
QuickLink™ DNA Ligation Kit
T4 DNA ligase is used for the joining of DNA molecules with compatible cohesive (sticky) termini, joining of blunt ended double stranded DNA molecules
Extracellular Vesicle Protocol
Best practices for standardizing and reducing the Extracellular Vesicle Preparation workflow, and eliminating contamination of EV preparations with dead-cell-derived vesicles.
Study: Comparison of MC-Media Pad® with Different Convenience Solution Products
Comparison of MC-Media Pad® with different convenience solution products for indicator organism testing
Enzymatic Assay of Superoxide Dismutase
Enzymatic Assay of Superoxide Dismutase
Enzymatic Assay of Trypsin Inhibitor
This technical article described the Enzymatic Assay of Trypsin Inhibitor.
Simple dialysis of Small Volume Samples using V Series Membranes
This article is a protocol for Simple dialysis of small volumes using V Series membranes
Polar Compound Retention using Aqueous Normal-Phase (ANP/HILIC) Chromatography
Polar Compound Retention using Aqueous Normal-Phase (ANP/HILIC) Chromatography
Determination of Headspace Volatiles in Distilled Spirits Using SPME Coupled to GC
In this article, SPME-GC was successfully used to identify many volatile compounds present in the headspace over several spirits.
HPLC Analysis of Aspirin and Salicylic Acid on Ascentis® Express RP-Amide
Separation of Acetylsalicylic acid, analytical standard; Salicylic acid, BioXtra, ≥99.0%
Determination of Bisphenol A in Drinking Water
A comprehensive sample preparation and analytical procedure was developed by Supelco for determining BPA in drinking water.
UHPLC Analysis Cannabinoids Ascentis Express
As the popularity of cannabis-infused products increases, there is a growing need to characterize the type and content of the cannabinoids found in the product. This application demonstrates the ability of the Ascentis Express C18 column to baseline resolve
Fast and Accurate LC-MS Analysis of Vitamin D Metabolites Using Ascentis® Express F5 HPLC Columns
Fast and Accurate LC-MS Analysis of Vitamin D Metabolites, Using Ascentis® Express F5 HPLC Columns from Supelco®
2-Hydroxyglutarates Analysis by LCMS
Reversed phase application for the analysis of S-2-hydroxyglutaric acids and R-2-hydroxyglutaric acids by chiral LCMS, using a CHIROBIOTIC® R Column.
HPLC Analysis of Dehydroascorbic Acid and Ascorbic Acid (Vitamin C) on SeQuant ZIC-HILIC
HPLC Analysis of Dehydroascorbic Acid and Ascorbic Acid (Vitamin C) on SeQuant ZIC-HILIC
LC/MS Analysis of Glyphosate and Metabolites on apHera™ NH2, 2 mm I.D. Column
LC/MS Analysis of Glyphosate and Metabolites on apHera™ NH2, 2 mm I.D. Column
Reliable DNA extraction from Whatman™ FTA cards
This study examined the yield and quality of DNA from samples applied to Whatman FTA cards, using five common methods of DNA extraction.
CRISPR Cas 9 Nuclease RNA-guided Genome Editing
Learn about CRISPR Cas9, what it is and how it works. CRISPR is a new, affordable genome editing tool enabling access to genome editing for all.
Protector RNase Inhibitor Protocol & Troubleshooting
The addition of Protector RNase Inhibitor to cell pellets is not recommended. Protector RNase Inhibitor as a 50kDa protein will most probably not permeate into intact cells. If the cell pellet is immediately frozen at -80 °C, this will be
Saliva DNA Extraction & WGA Amplification Protocol
Whole Genome Amplification can be performed on DNA extracted in many ways. We offer many products for DNA extraction, including the GenElute™ Blood Genomic DNA Kit, GenElute Mammalian Genomic DNA Miniprep Kit and the GenElute Plant Genomic DNA M iniprep.
Plant DNA Extraction & WGA Amplification Protocol
GenomePlex® Whole Genome Amplification has been used to amplify genomic DNA from soybean, corn, tomato, purple coneflower, and ginseng.
Nucleic Acid Sample Preparation Methodology
Spectrophotometry can be used to estimate DNA or RNA concentration and to analyze the purity of the preparation.
FACS Titration of Lentivirus Protocol
FACS (Fluorescence-Activated Cell Sorting) provides a method for sorting a mixed population of cells into two or more groups, one cell at a time, based on the specific light scattering and fluorescence of each cell. This method provides fast, objective
HPLC Analysis of Immunoglobulin G (IgG) on Zenix® SEC-150 versus Zenix® SEC-300, Effect of Pore Size on Resolution
HPLC Analysis of Immunoglobulin G (IgG) on Zenix® SEC-150 versus Zenix® SEC-300, Effect of Pore Size on Resolution
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