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CRISPR/Cas9 Products and Services
Synonym: CRISPR, CRISPRs, Cas9, Crispr RNA, Crispr/Cas System, Synthetic CRISPR, crRNA, tracrRNA
MISSION® TRC3 Human LentiORF Collection
MISSION® TRC3 Human LentiORF Collection; Synonym: Blasticidin ORF, Entry ORF, Gateway Cloning, Gene Expression, Gene Overexpression, Gene Validation, LentiORF, MISSION® TRC3 Human LentiORF, MISSION® TRC3 ORF, Open Reading Frame, Protein Expression, Puromycin ORF, Rescue Experiments, TRC3; find null-ORF MSDS, related
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Modulated Gene Expression in 3D Cell Culture: Implications for Drug Discovery
Introduction of the modulation in expression profile for some genes and proteins when comparing 2D versus 3D cell culture conditions.
MaxGel™ ECM Hydrogel: An In Vitro Human Cell Derived Basement Membrane Extract for 3D Cell Culture Applications
Human cell derived basement membrane extract (BME) hydrogel for 3D cell culture of primary cell, stem cell and xenograph cancer models.
Nanofiber Scaffold Technology Validated in a Historic Human Implant
Technical Article on Nanofiber Scaffold Technology Validated in a Historic Human Implant.
Cyst Formation of MDCK Cells in TrueGel3D™ Hydrogels
TrueGel3D synthetic hydrogel can be used to study cyst formation of MDCK cells.
Primary Airway Epithelial Cells and Growth Media
Primary human airway epithelial cells and serum-free growth media are optimized for the in vitro cultivation of epithelial cells from large air passages. Protocols for handling, cell passaging, media, and product use.
Naive Pluripotent Stem Cell Culture in an Inhibitor Based 2i/LIF Containing Serum-Free and Feeder-Free Stem Cell Media
Naive pluripotent stem cells are located within the epiblast of mature blastocysts. These primitive “ground-state” cells may be cultured in vitro using specialized media and small molecule inhibitors.
PromoCell® Primary Cell Pellets
Primary cells from PromoCell are available in pelleted form for research applications like gene expression analysis, and cell/protein characterization experiments.
Primary Human Melanocytes and Growth Media
Primary Human Epidermal Melanocytes and growth media optimized for in vitro cultivation of human melanocytes, human pigment epithelial cells, melanoma cells, and neuronal cells. Protocols for handling, cell passaging, media, and product use.
Single Transfection of a Synthetic Polycistronic Self-replicative RNA Yields High Numbers of Transgene-free Human iPSCs
The Simplicon™ RNA Reprogramming Technology is a next generation reprogramming system that uses a single synthetic, polycistronic self-replicating RNA strand engineered to mimic cellular RNA to generate human iPS cells.
ReNcell® Human Neural Progenitors: Renewable and Consistent Human Neural Stem Cell Lines that Differentiate into Functional Neurons
ReNcell neural progenitors are immortalized human neural stem cell lines that can differentiate into neurons, astrocytes sand oligodendrocytes.
Hepatotoxicity/Cytotoxicity Assays
The hepatotoxicity/cytotoxicity assays provide a means to measure cell death due to the cytotoxic effect of a test compound. We provide hepatotoxicity/cytotoxicity assays for all small molecule formulations such as pharmaceuticals, industrial chemicals and consumer products.
Fluorescent Microparticles
Fluorescent Microparticles in Biochemistry. Fluorescently labeled particles are useful as standards in flow cytometry, confocal laser scanning microscopy, and with light scattering instruments.
Fluorescent Live Cell Imaging of Cytoskeleton Structure Proteins using LentiBrite™ Lentiviral Biosensors
High titer lentiviral particles including beta-actin, alpha-tubulin and vimentin used for live cell analysis of cytoskeleton structure proteins.
Real-time imaging of adherent and non-adherent cell interactions: utility of an automated microfluidic trap platform to recapitulate in vivo cell culture microenvironment
This page describes novel microfluidic approaches to trap two different cell types in precise locations in order to image and study their interactions over time.
Apoptosis Assays
Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.
Zinc Finger Nuclease References
Zinc Finger Nuclease References: Human, Rat, Mouse, Zebrafish, Plasmodium, Xenopus, Rainbow Trout, Mosquito, Pig, Rabbit, Cattle, CHO, ZFN Technology, Reviews, Posters, Label License
Detection of Endogenous Pathway Activity in Novel Reporter Cell Lines
Our company presents an article on the Detection of Endogenous Pathway Activity in Novel Reporter Cell Lines
CRISPR on the Brain? Standardized Functional Genomics Tools Advance Neuroscience
Deciphering the genetics of neuroscience has always been challenging. Heterogeneous tissue microenvironments, complex genetic interactions and phenotypes, lack of model systems that accurately mimic the human brain transcriptome - not to mention the blood-brain barrier - make genetic perturbation analysis
Lentivirus Cell Line
Using lentivirus as a means to deliver shRNAs has become standard practice in many labs exploring RNAi.
Viral Invaders
Protection at the genetic level – a new line of defense against contamination in biopharmaceutical manufacturing.
Genome Engineering with CompoZr Custom Zinc Finger Nucleases (ZFNs)
Genome Editing is a powerful technique that can be used to elucidate gene function and the genetic basis of disease. Watch our video.
Mycoplasma Detection and Elimination
Detect mycoplasma contamination in cell cultures three ways: culture test, DNA stain test, or mycoplasma PCR test. Mycoplasma elimination kits rid cultures of contamination.
Primer Optimization Protocol Using Temperature Gradient
Gradient PCR for assay optimization is to determine the optimum annealing temperature (Ta) of the primers by testing identical reactions containing a fixed primer concentration, across a range of annealing temperatures.
Amplification of Damaged DNA with Restorase DNA Polymerase (R1028)
Method for amplification of DNA from damaged DNA sources. Particularly useful for DNA extracted from old samples.
qPCR Gene Expression Using Probe Detection
The most common application for qPCR is the measurement of a gene transcript or copy number quantity relative to one or more reference genes using probe detection.
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