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Primary cells from PromoCell are available in pelleted form for research applications like gene expression analysis, and cell/protein characterization experiments.

Primary Human Melanocytes and Growth Media

Primary Human Epidermal Melanocytes and growth media optimized for in vitro cultivation of human melanocytes, human pigment epithelial cells, melanoma cells, and neuronal cells. Protocols for handling, cell passaging, media, and product use.

Fluorescent Live Cell Imaging of Cytoskeleton Structure Proteins using LentiBrite™ Lentiviral Biosensors

High titer lentiviral particles including beta-actin, alpha-tubulin and vimentin used for live cell analysis of cytoskeleton structure proteins.

Real-time imaging of adherent and non-adherent cell interactions: utility of an automated microfluidic trap platform to recapitulate in vivo cell culture microenvironment

This page describes novel microfluidic approaches to trap two different cell types in precise locations in order to image and study their interactions over time.

Apoptosis Assays

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

Viral Invaders

Protection at the genetic level – a new line of defense against contamination in biopharmaceutical manufacturing.

Preparing Cells for Electroporation

Purification of Serum Peptides for MALDI Mass Spectrometry with Amicon® Ultra Centrifugal Filters

Biomarkers play an essential role in the drug discovery and development process.

LC-UV-MS Method Development for Antibody Drug Conjugates Using a Non-Toxic ADC-Mimic

SigmaMab Antibody Drug Conjugate Mimic, is a non-toxic drug mimic utilized as a standard for mass spectrometry and high performance liquid chromatography.

Rapid DNA Ligation Kit Protocol

DNA Ligation Protocol

The cloning process requires the ligation of linear DNA into a cloning vector. This ability to join fragments of DNA through recombinant technology is essential for many basic experiments in biotechnology.

DIG Gel Mobility Shift Assay Troubleshooting

To prevent nonspecific binding of the analyzed protein or extract to the DNA fragment or oligonucleotide, nonspecific competitor nucleic acid is added to the binding reaction.

ARGET ATRP: Procedure for PMMA Polymer Brush Growth

We presents an article about ARGET ATRP, and its procedure for PMMA polymer brush growth. Surface preparation before polymer brush growth consists of two steps: surface cleaning and initiator monolayer deposition.

QuickLink™ DNA Ligation Kit

T4 DNA ligase is used for the joining of DNA molecules with compatible cohesive (sticky) termini, joining of blunt ended double stranded DNA molecules

ELISA Protocols

This article offers 4 popular ELISA protocols: Sandwich ELISA protocol, Phosphorylation Assay Procedure, EIA Assay Procedure, & Cell-based Assay Procedure.

Polymer Semiconductors for Intrinsically Stretchable Organic Transistors

Intrinsically stretchable active layers for organic field-effect transistors (OFET) are discussed. Polymer structural modification & post-polymerization modifications are 2 methods to achieve this.

Self-Assembled Monolayers: Advantages of Pure Alkanethiols

We presents an article regarding Self-Assembled Monolayers: Advantages of Pure Alkanethiols.

New Conducting and Semiconducting Polymers for Plastic Electronics

In the emerging field of organic printable electronics, such as OLEDs and organic photovoltaics (OPVs), there is a significant need for improved organic conducting and semiconducting materials. This paper reports our recent progress in two fields: 1) the development of

Substituting Strat-M® Membrane for Human Skin in Evaluating Effect of Encapsulation on Diffusion of Sunscreen Formulations

Understand how a Strat-M synthetic membrane model can be an alternative to human or animal skin when screening for effectiveness of encapsulation on reducing transdermal diffusion of skin care actives

Traditional HPLC is Incapable of Reducing Cross Contamination of Custom Next-Generation Sequencing Adapters to Acceptable Levels

he development of next-generation massively parallel sequencing technologies (NGS), including the Roche 454™ Genome Sequencer FLX Instrument, the Illumina Genome Analyzer, Life Technology’s Ion Torrent™ Personal Genome Machine, and the Pacific Biosciences RS have revolutionized genomic and genetic research, significantly

Carcinogenesis and Epigenetics

Cancer research has revealed that the classical model of carcinogenesis, a three step process consisting of initiation, promotion, and progression, is not complete.

Acute and Chronic Inflammation: Microglia in Neuroprotection and Neurodegeneration

The term neurodegeneration characterizes a chronic loss of neuronal structure and function leading to progressive mental impairments.

Acetylcholine Receptors (Nicotinic)

Sigma-Aldrich offers many products related to nicotinic acetylcholine receptors for your research needs.

A Tissue Dictionary for Normal and Cancer Tissues: A complimentary tool for the Human Protein Atlas

Autophagy Mitophagy in Tumor Cell Survival Death

Autophagy is a highly regulated process that is involved in cell growth, development, and death. In autophagy cells destroy their own cytoplasmic components in a very systematic manner and recycle them.

Tec

Tec kinases represent the second largest family of nonreceptor tyrosine kinases and are activated in response to cellular stimulation by antigen receptors, integrins, growth factors, cytokines and G protein-coupled receptors.

Epigenetic Mechanisms in Cancer Pathogenesis

This page reviews some of our newest and most innovative technologies and their specific applications toward cancer research. One is reminded of how complex the disease of cancer is, and how difficult it is to identify one topic that is

Properties of Mononuclear Cells Isolated by the Ficoll-Paque Method

The effects of the mononuclear cell separation method described by Bøyum procedure are noted below, since research situations may arise in which they are significant.

Ginseng (Panax quinquefolius) Augments Doxorubicin-Induced Inhibition of Human Breast Cancer Cell Proliferation and Tumor Growth: Mechanisms of Action

Ginseng root extract inhibits the proliferation of human cancer cells in vitro, including those that represent many common cancers, such as breast cancer. Ginseng extracts contain over 20 different ginsenosides, saponin glycosides that are biologically active constituents of ginseng. Several

Glycine Receptors

The amino acid glycine is a major inhibitory neurotransmitter in the vertebrate CNS. Glycinergic synapses are particularly abundant in spinal cord and brain stem, but are also found in higher brain regions including the hippocampus. The inhibitory actions of glycine

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